⮝ Full datasets listing
PXD082900
PXD082900 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Bio-propofol affinity profiling implicates extracellular Slit2 in propofol responsiveness |
| Description | Propofol (2,6-diisopropylphenol) is a widely used intravenous anaesthetic with biological effects extending beyond transient loss of consciousness, including actions on mitochondrial function, immune responses, neuronal differentiation, axonal transport and synaptic plasticity. Every propofol-binding protein identified to date is membrane-embedded or intracellular, and whether the propofol scaffold engages extracellular signaling molecules is unknown. Using a hypnotically active biotinylated propofol analogue, we performed affinity enrichment from mouse brain followed by label-free LC–MS/MS, and identified Slit2, a secreted axon-guidance cue. Bio-propofol enriched endogenous Slit2 from brain lysate and Slit2–FLAG from conditioned medium, and free propofol competed this recovery. Docking analyses identified a favorable cavity on the concave face of the Robo-binding LRR2 domain. In Caenorhabditis elegans, slt-1(ok255) mutants were markedly resistant to propofol-induced paralysis. These convergent data identify Slit2 as a candidate extracellular propofol-associated protein and link Slit signaling to organismal propofol responsiveness. |
| HostingRepository | iProX |
| AnnounceDate | 2026-08-19 |
| AnnouncementXML | Submission_2026-08-20_07:15:04.425.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Tiansi Cui |
| SpeciesList | scientific name: Mus musculus; NCBI TaxID: 10090; |
| ModificationList | acetylated residue; methionine oxidation with neutral loss of 64 Da |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-08-20 07:14:25 | ID requested | |
| ⏵ 1 | 2026-08-20 07:15:04 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: propofol, LC-MS/MS |
Contact List
| Weike Ji | |
|---|---|
| contact affiliation | Huazhong University of Science and Technology |
| contact email | Weike_Ji1985@163.com |
| lab head | |
| Tiansi Cui | |
| contact affiliation | Huazhong University of Science and Technology |
| contact email | omo0721@qq.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
