⮝ Full datasets listing
PXD082899
PXD082899 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteomic and phosphoproteomic profiling of mouse granulosa cells during in vitro luteinization (0h, 48h, 96h) |
| Description | This project investigates the dynamic changes in global protein expression and phosphorylation modifications during the in vitro luteinization of human granulosa cells. Granulosa cells were cultured and harvested at three distinct time points: 0 hrs, 48 hrs, and 96 hrs. Protein extracts were subjected to tryptic digestion and analyzed by high-resolution LC-MS/MS to generate both comprehensive proteomic and phosphoproteomic datasets. This time-series profiling aims to decipher the molecular signaling pathways and regulatory networks governing granulosa cell luteinization, including steroidogenesis and cellular differentiation. Bioinformatic analysis of this time-series dataset identified the MAPK signaling pathway as a prominently regulated hub during luteinization progression. In the subsequent validation phase, granulosa cells were treated with U0126, a specific MEK1/2 inhibitor, under identical culture conditions, and samples at matched time points were subjected to the same proteomic and phosphoproteomic workflows. Comparative analysis between control and U0126-treated datasets will systematically characterize MAPK-dependent regulatory networks during in vitro luteinization. |
| HostingRepository | iProX |
| AnnounceDate | 2026-08-19 |
| AnnouncementXML | Submission_2026-08-20_07:12:54.497.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Shen Zhang |
| SpeciesList | scientific name: Mus musculus; NCBI TaxID: 10090; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Eclipse |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-08-20 07:12:14 | ID requested | |
| ⏵ 1 | 2026-08-20 07:12:55 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Granulosa cells, luteinization, Proteomics, Phosphoproteomics In vitro culture, MAPK |
Contact List
| Shen Zhang | |
|---|---|
| contact affiliation | NHC Key Laboratory of Human Stem Cell and Reproductive Engineering, Xiangya School of Basic Medical Sciences, Central South University |
| contact email | szhang231@126.com |
| lab head | |
| Shen Zhang | |
| contact affiliation | NHC Key Laboratory of Human Stem Cell and Reproductive Engineering, Xiangya School of Basic Medical Sciences, Central South University |
| contact email | szhang231@126.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
