⮝ Full datasets listing
PXD082827
PXD082827 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Quantitative proteomic profiling of endogenous oISG15-associated proteins during bluetongue virus infection |
| Description | ISG15 is an interferon-induced ubiquitin-like protein that exerts diverse functions during viral infection. We previously reported that ovine ISG15 (oISG15) promoted Bluetongue virus (BTV) replication by stabilizing viral NS1 and VP4 in an ISGylation-independent manner, although the mechanism remained unknown. Here, using the generated an-ti-oISG15 antibody, we performed immunoprecipitation (IP) coupled with label-free LC-MS/MS to characterize the oISG15-associated proteome in BTV-infected and uninfected cells. The results showed that BTV infection markedly remodeled the oISG15 association landscape, with decreased enrichment of several autophagy- and trafficking-related proteins and increased enrichment of mitochondrial metabolic proteins in oISG15 im-munoprecipitates. Functionally, oISG15 overexpression induced modest alterations in autophagy-related markers in uninfected cells yet attenuated these markers’ abundance during BTV infection. Together, this study revealed BTV-associated changes in the oISG15-associated proteome that coincided with alterations in autophagy-related markers. |
| HostingRepository | iProX |
| AnnounceDate | 2026-08-18 |
| AnnouncementXML | Submission_2026-08-19_01:34:31.660.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Di Kang |
| SpeciesList | scientific name: Ovis aries; NCBI TaxID: 9940; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | timsTOF Pro |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-08-19 01:31:17 | ID requested | |
| ⏵ 1 | 2026-08-19 01:34:32 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: ISG15, Bluetongue virus, Proteomics, Autophagy, Mitochondrial metabolism |
Contact List
| Di Kang | |
|---|---|
| contact affiliation | Gansu Agricultural University |
| contact email | kangd@gsau.edu.cn |
| lab head | |
| Di Kang | |
| contact affiliation | Gansu Agricultural University |
| contact email | kangd@gsau.edu.cn |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
