⮝ Full datasets listing
PXD082732
PXD082732 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Identification of candidate markers for the isolation of neuron-specific extracellular vesicles |
| Description | Extracellular vesicles (EVs) are present in biofluids and may be useful biomarkers to non-invasively monitor brain cells, but isolating cell type-specific EVs remains challenging. Here, we introduce an approach to immuno-isolate EVs from neurons (and glia) based on protein surface markers. We first selected transmembrane proteins suitable as cell type-specific EV markers based on gene expression and EV proteomics data. We developed a high-purity EV isolation method and cataloged the proteins present on EVs in human cerebrospinal fluid (CSF) and plasma. We then computationally identified cell-type specific candidate markers for isolating EVs from neurons, astrocytes, oligodendrocytes, and microglia. Using ultrasensitive immunoassays against several predicted neuron-specific proteins, we confirmed one marker, NRXN3, as present on EVs in CSF and plasma. Finally, we developed efficient EV immuno-isolation methods and applied them to isolate NRXN3+ EVs. Our work provides a general methodology for the isolation of cell-type specific EVs, advancing the goal of using EVs to study brain disease. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-08-18 |
| AnnouncementXML | Submission_2026-08-18_10:27:08.545.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Bogdan Budnik |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | Oxidation; Deamidated; Carbamidomethyl |
| Instrument | Orbitrap Astral |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-08-17 15:08:11 | ID requested | |
| ⏵ 1 | 2026-08-18 10:27:09 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Extracellular vesicles, EVs, exosomes, DatasetType:Proteomics |
Contact List
| David R Walt | |
|---|---|
| contact affiliation | Harvard Medical School |
| contact email | dwalt@bwh.harvard.edu |
| lab head | |
| Bogdan Budnik | |
| contact affiliation | Harvard University |
| contact email | Bogdan.Budnik@wyss.harvard.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v14/MSV000102882/ |
