PXD082569

PXD082569 is an original dataset announced via ProteomeXchange.

Title	LC–MS/MS identification of DHHC12-2CR-associated proteins under amino acid-replete and amino acid-deprived conditions
Description	This dataset was generated to identify proteins that associate with DHHC12 in response to amino acid deprivation. HEK293T cells expressing Flag-tagged DHHC12-2CR were cultured in complete or amino acid-deficient medium for 3 h and subjected to Flag co-immunoprecipitation. The eluates were separated by SDS–PAGE, and an approximately 150-kDa band enriched under amino acid-deficient conditions was excised and analyzed by LC–MS/MS. COPA was identified as a candidate amino acid deprivation-enhanced DHHC12-interacting protein and was subsequently validated by co-immunoprecipitation and immunoblotting. These data support the involvement of COPA in the nutrient-dependent regulation of DHHC12 ubiquitination and degradation.
HostingRepository	PRIDE
AnnounceDate	2026-09-07
AnnouncementXML	Submission_2026-09-06_21:04:03.034.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shi Qing
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	ubiquitination signature dipeptidyl lysine; phosphorylated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2026-08-14 01:24:18	ID requested
⏵ 1	2026-09-06 21:04:03	announced

Dataset with its publication pending

submitter keyword: DHHC12, nutrient sensing, co-immunoprecipitation, CRL4 E3 ubiquitin ligase, ubiquitination, HEK293T cells, protein–protein interaction, protein degradation, IP–MS, amino acid deprivation, COPA, LC–MS/MS

Tongjin Zhao
contact affiliation	Institute of Metabolism and Integrative Biology, School of Life Sciences, Fudan University, Shanghai, China
contact email	Zhaotj@fudan.edu.cn
lab head
Shi Qing
contact affiliation	Fudan University
contact email	m17321005352@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD082569
     1. Label: PRIDE project
     2. Name: LC–MS/MS identification of DHHC12-2CR-associated proteins under amino acid-replete and amino acid-deprived conditions