PXD082515

PXD082515 is an original dataset announced via ProteomeXchange.

Title	Whale Müller glia create a permissive glial environment that supports retinal ganglion cell survival and neurite outgrowth
Description	The adult central nervous system exhibits a limited capacity for neuronal regeneration, with retinal ganglion cells being particularly refractory to repair. Although this limitation is frequently attributed to neuron-intrinsic constraints, the contribution of the extracellular glial environment remains incompletely understood. Here, we describe a Müller glial phenotype derived from large baleen whales that supports retinal ganglion cell survival and neurite extension. Using cross-species co-culture and conditioned-medium approaches, we show that whale Müller glia enhance neuronal viability and neurite elongation compared with Müller glia from pigs and rats. Quantitative proteomic profiling and functional annotation suggested species-dependent differences in conditioned-medium composition. Whale Müller glia-conditioned medium contained a broader repertoire of proteins associated with neuroprotection and neurite growth and a lower representation of proteins linked to inhibitory regulation than pig Müller glia-conditioned medium. Among the identified candidates, mesencephalic astrocyte-derived neurotrophic factor (MANF) showed greater abundance in whale conditioned medium, whereas fibronectin leucine-rich transmembrane protein 2 (FLRT2), a molecule associated with axon repulsion, was detected in pig conditioned medium. Functional perturbation experiments showed that FLRT2 suppression enhanced neurite outgrowth in a subset of cells within the RGC-enriched cultures when combined with MANF, but only in the presence of Müller glia, suggesting a glia-dependent effect. Overall, this study identifies species-associated differences in Müller glia-conditioned media and candidate molecular signals that may contribute to retinal neuronal survival and neurite outgrowth.
HostingRepository	PRIDE
AnnounceDate	2026-09-02
AnnouncementXML	Submission_2026-09-02_04:15:09.757.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stefanie Hauck
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: NEWT:9825;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2026-08-13 10:48:05	ID requested
⏵ 1	2026-09-02 04:15:11	announced

Dataset with its publication pending

submitter keyword: Whale, Neuron survival, Müller glia, Neurite outgrowth, Retinal ganglion cells

Elena Vecino
contact affiliation	Experimental Ophthalmo-Biology Group, Department of Cell Biology and Histology, University of the Basque Country UPV/EHU, Leioa, Spain
contact email	elena.vecino@ehu.eus
lab head
Stefanie Hauck
contact affiliation	Research Unit Protein Science, Helmholtz Zentrum München
contact email	hauck@helmholtz-muenchen.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD082515
     1. Label: PRIDE project
     2. Name: Whale Müller glia create a permissive glial environment that supports retinal ganglion cell survival and neurite outgrowth