PXD082313

PXD082313 is an original dataset announced via ProteomeXchange.

Title	RcrR: A Multifunctional Transcriptional Regulator Orchestrating the cariogenic virulence of Streptococcus mutans
Description	Streptococcus mutans is the primary etiological agent of dental caries, with its pathogenicity depending on biofilm formation, acidogenicity, and acid tolerance. RcrR, a conserved MarR-family transcription factor, has been linked to stress adaptation; however, its direct role in regulating the core cariogenic virulence and the underlying mechanisms remain unclear. Here, we systematically investigated RcrR's contribution to cariogenic virulence using isogenic wild-type, ΔrcrR deletion mutant, and complemented strains. We assessed bacterial growth, biofilm architecture, acid tolerance, and H⁺-ATPase activity. To delineate the global regulatory network, we integrated untargeted metabolomics with data-independent acquisition (DIA)-based quantitative proteomics. Deletion of rcrR impaired bacterial growth, resulted in a structurally loose biofilm with significantly reduced water-insoluble extracellular polysaccharides (EPS) and extracellular DNA (eDNA), and markedly downregulated the transcription of glucosyltransferase genes gtfB, gtfC, and gtfD. Under acidic conditions (pH 5.5), the ΔrcrR mutant showed substantially reduced acidogenicity and aciduricity, accompanied by significantly diminished H⁺-ATPase activity. Multi-omics analyses revealed that rcrR deletion depleted critical glycolytic intermediates, most notably fructose-1,6-bisphosphate (FBP), and broadly suppressed the expression of proteins involved in carbohydrate metabolism, ABC transport systems, and quorum sensing pathways. Our findings demonstrate that RcrR functions as a central regulator coordinating energy metabolism, nutrient transport, and cell signaling to drive the full spectrum of S. mutans cariogenic virulence. This identifies RcrR as a promising molecular target for the development of novel preventive and therapeutic strategies against dental caries.
HostingRepository	iProX
AnnounceDate	2026-08-07
AnnouncementXML	Submission_2026-08-06_18:50:27.693.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xuan He
SpeciesList	scientific name: Streptococcus mutans; NCBI TaxID: 1309;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2026-08-06 18:50:10	ID requested
⏵ 1	2026-08-06 18:50:28	announced

Dataset with its publication pending

submitter keyword: Streptococcus mutans, RcrR, Biofilm, Acid tolerance, Metabolomics, Proteomics

Lijun Huo
contact affiliation	Yunnan Provincial Stomatology Hospital ,Kunming Medical University
contact email	huolijun1039@kmmu.edu.cn
lab head
Xuan He
contact affiliation	Yunnan Provincial Stomatology Hospital ,Kunming Medical University
contact email	hx707400@163.com
dataset submitter

iProX dataset URI