PXD082260

PXD082260 is an original dataset announced via ProteomeXchange.

Title	Identification of FAK-interacting proteins by LC-MS/MS from silver-stained gel bands
Description	FAK (focal adhesion kinase) is a key regulator of cell adhesion and signaling. To identify potential FAK-interacting proteins, we expressed FLAG-tagged FAK (FAK-FLAG) or empty vector (EV) control in HEK293T cells, followed by anti-FLAG affinity pull-down. The immunoprecipitated complexes were resolved by SDS-PAGE and visualized by silver staining. Specific protein bands enriched in the FAK-FLAG lane compared to the EV control were excised, subjected to in‑gel trypsin digestion, and analyzed by LC‑MS/MS. Protein identification was performed, revealing their potential association with FAK. This dataset provides the raw mass spectrometry data and identification results for these candidate interactors.
HostingRepository	iProX
AnnounceDate	2026-08-06
AnnouncementXML	Submission_2026-08-06_01:47:08.488.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhi Ying
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 240

Revision	Datetime	Status	ChangeLog Entry
0	2026-08-06 01:46:47	ID requested
⏵ 1	2026-08-06 01:47:08	announced

Dataset with its publication pending

submitter keyword: FAK, silver-stained SDS-PAGE, protein-protein interaction

Ting Dong
contact affiliation	Institute of Medicinal Biotechnology (IMB), Chinese Academy of Medical Sciences & Peking Union Medical College
contact email	tingdong2025@pumc.edu.cn
lab head
Zhi Ying
contact affiliation	Shandong University
contact email	wangzhiying7078@163.com
dataset submitter

iProX dataset URI