PXD082164

PXD082164 is an original dataset announced via ProteomeXchange.

Title	In depth mapping of the neuroblastoma secretome reveals an immunosuppressive secretory phenotype inhibiting effective T cell responses through extracellular vesicles
Description	Neuroblastoma is a pediatric solid tumor that remains a challenge for immunotherapy. This limited efficacy is attributed to immunomodulatory factors secreted within the neuroblastoma tumor microenvironment (TME), which suppress T-cell function promoting tumor survival and metastasis. To identify neuroblastoma derived immunomodulatory factors, we isolated and characterized the secretomes of 16 patient-derived tumoroids. These secretomes suppressed T-cell activation, proliferation, and cytotoxicity to varying degrees, demonstrating the presence of factors capable of modulating immune cell function. We therefore profiled the secretomes using LC-MS/MS and identified neuropeptide Y (NPY), together with several previously reported immunomodulatory factors, as significantly enriched in the most suppressive tumoroids. Although neither direct nor indirect effects of NPY on T-cell could be validated, NPY abundance correlated with extracellular vesicle (EV) secretion. This finding suggests that NPY is a biomarker of EV secretion rather than a direct mediator of T-cell suppression and may also indicate the immunosuppressive status of the TME.
HostingRepository	PRIDE
AnnounceDate	2026-08-17
AnnouncementXML	Submission_2026-08-16_23:07:08.258.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Elisavet Kalaitsidou
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2026-08-04 14:25:02	ID requested
⏵ 1	2026-08-16 23:07:08	announced

Dataset with its publication pending

submitter keyword: proteomics, T-cells, extracellular vesicles, immunosuppression,Neuroblastoma, secretome

Wei Wu
contact affiliation	Singapore Immunology Network (SIgN), Agency for Science, Technology and Research (A*STAR), 8A Biomedical Grove, Immunos, Singapore 138648, Republic of Singapore; Department of Pharmacy and Pharmaceutical Sciences, National University of Singapore, 117543, Singapore, Singapore;
contact email	Wu_Wei@a-star.edu.sg
lab head
Elisavet Kalaitsidou
contact affiliation	1. National University of Singapore, 2. Singapore Immunology Network (SIgN), Agency for Science, Technology and Research (A*STAR)
contact email	elisavet.k@u.nus.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD082164

PRIDE project URI

• PRIDE
  1. PXD082164
     1. Label: PRIDE project
     2. Name: In depth mapping of the neuroblastoma secretome reveals an immunosuppressive secretory phenotype inhibiting effective T cell responses through extracellular vesicles