PXD082145

PXD082145 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomic analysis of ribosome-associated proteins following human-like stressors in Cryptococcus neoformans
Description	During human infection, Cryptococcus neoformans can disseminate to the central nervous system causing often fatal cryptococcal meningitis in individuals with compromised immunity. To acutely adapt to host microenvironments, C. neoformans employs mechanisms to reprogram its translatome to provide cells with tools to survive the stressors of the human host. The identities and functions of ribosome-associating factors that may modulate this process in C. neoformans remain unknown. We hypothesized that following host-related stressors, specific proteins associate with ribosomes and influence stress adaptation in C. neoformans. Utilizing a proteomic approach, termed RiboPROT, to analyze the protein composition of mRNA-associated ribosomes from cells grown unstressed (30˚C), thermally stressed (shifted to 37°C), and oxidatively stressed (shifted to 2 mM H2O2) conditions. We identified unique factors enriched with actively translating ribosomes during host-related stressors
HostingRepository	PRIDE
AnnounceDate	2026-09-08
AnnouncementXML	Submission_2026-09-08_10:31:17.508.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	John Panepinto
SpeciesList	scientific name: Cryptococcus neoformans; NCBI TaxID: NEWT:5207;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2026-08-04 08:00:10	ID requested
⏵ 1	2026-09-08 10:31:18	announced

Dataset with its publication pending

submitter keyword: Fungal pathogen, polysomes, Translation

John Panepinto
contact affiliation	University at Buffalo, SUNY
contact email	jcp25@buffalo.edu
lab head
John Panepinto
contact affiliation	University at Buffalo
contact email	jcp25@buffalo.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD082145
     1. Label: PRIDE project
     2. Name: Quantitative proteomic analysis of ribosome-associated proteins following human-like stressors in Cryptococcus neoformans