PXD082056

PXD082056 is an original dataset announced via ProteomeXchange.

Title	The quantification of Cysteine Sulfonation Using Resin-Assisted Capture Oxidation and a Spike-in Internal Standard Strategy
Description	Cysteine, despite its low abundance, demonstrates significant functional versatility. Its thiol group (–SH) can undergo sequential oxidation by reactive oxygen species (ROS), progressing through sulfenation (–SOH) and sulfination (–SO₂H) to ultimately form irreversible sulfonation (–SO₃H). Sulfonation introduces a substantial, negatively charged moiety that modifies the residue's charge distribution, steric properties, and local interactions, consequently impacting protein conformation and function. This modification serves as a biomarker for oxidative stress and is linked to neurodegenerative diseases, myocardial ischemia/reperfusion injury, genetic deficiencies, and the aging process. Existing enrichment and quantification methodologies can only identify a restricted number of sulfonation sites. Our method, utilizing a magnetic resin functionalized with pyridyl disulfide groups, facilitates the efficient enrichment of thiol-containing peptides. Following release, these peptides are oxidized to produce sulfonated peptides, which serve as internal standards. This, in conjunction with dimethyl labeling, permits large-scale quantification of sulfonation.
HostingRepository	iProX
AnnounceDate	2026-08-02
AnnouncementXML	Submission_2026-08-03_00:35:04.976.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jinlong Wang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	L-cysteic acid (L-cysteine sulfonic acid)
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2026-08-03 00:32:19	ID requested
⏵ 1	2026-08-03 00:35:05	announced

Dataset with its publication pending

submitter keyword: Cysteine Sulfonation, Resin-Assisted Capture Oxidation, Spike-in Internal Standard, Oxidative Damage, Dimethyl labeling

Jinlong Wang
contact affiliation	Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences
contact email	jinlongwang@ucas.ac.cn
lab head
Jinlong Wang
contact affiliation	Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences
contact email	jinlongwang@ucas.ac.cn
dataset submitter

iProX dataset URI