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PXD082054
PXD082054 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | iTraq proteomics of SARM1-OE/HEK293 cells |
| Description | SARM1 (sterile alpha and Toll/interleukin-1 receptor motif-containing protein 1) is an inducible NAD-consuming enzyme that drives axon degeneration and a form of non-apoptotic cell death termed sarmoptosis. Using SARM1-overexpressing HEK293 cells, we previously showed that the cell-permeant NMN mimetic CZ-48 activates SARM1, depletes cellular NAD and ATP, and induces regulated, propidium iodide (PI)-positive, non-apoptotic cell death that is potently suppressed by the HSP70-family ATP-competitive inhibitor VER-155008 (VER). To investigate the proteome-wide changes underlying CZ-48-induced SARM1 activation and VER-mediated rescue, we performed iTRAQ-based quantitative proteomics on SARM1-overexpressing HEK293 cells treated with vehicle control, 20 μM VER alone, 100 μM CZ-48 alone, or CZ-48 plus VER for 16 h, with biological replicates per group. This dataset was used to identify CZ-48-responsive proteins whose abundance changes were reversed by VER co-treatment, focusing on HSP70/chaperone-related proteostasis proteins, mitochondrial and metabolic proteins, and cell-death-related proteins, in order to elucidate mechanisms of SARM1-driven ATP collapse and mitochondrial dysfunction during sarmoptosis. |
| HostingRepository | iProX |
| AnnounceDate | 2026-08-01 |
| AnnouncementXML | Submission_2026-08-03_17:49:38.190.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Linru Shen |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | TripleTOF 5600 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-08-03 00:17:50 | ID requested | |
| ⏵ 1 | 2026-08-03 17:49:38 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: SARM1, VER-155008, CZ-48, iTRAQ |
Contact List
| Lei Fang | |
|---|---|
| contact affiliation | Nanjing University |
| contact email | njfanglei@nju.edu.cn |
| lab head | |
| Linru Shen | |
| contact affiliation | Nanjing University |
| contact email | 502024350026@smail.nju.edu.cn |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
