PXD081994

PXD081994 is an original dataset announced via ProteomeXchange.

Title	Proteomic Insights into Hepatic Responses to High Soybean Meal Inclusion with Citric Acid Supplementation in Hybrid Grouper (Epinephelus fuscoguttatus × Epinephelus lanceolatus) Juveniles
Description	Fishmeal replacement with plant protein is an important strategy for sustainable aquaculture, although high soybean meal inclusion may impair growth and metabolism in carnivorous fish. This study investigated the hepatic proteomic responses of juvenile hybrid grouper (Epinephelus fuscoguttatus × Epinephelus lanceolatus) fed diets containing 30% or 40% soybean meal, with or without 3% citric acid supplementation, compared with a fishmeal control diet. Liver proteins were extracted and analyzed using label-free LC-MS/MS on a Thermo Scientific Q Exactive Plus mass spectrometer. A total of 1,544 proteins were identified, and differential protein abundance, Gene Ontology enrichment, KEGG pathway analysis, and protein-protein interaction network analyses were performed to investigate metabolic adaptation associated with dietary treatments. The dataset provides molecular insights into hepatic responses to soybean meal replacement and the effect of citric acid supplementation on nutrient utilization and energy metabolism in hybrid grouper.
HostingRepository	PRIDE
AnnounceDate	2026-08-17
AnnouncementXML	Submission_2026-08-16_16:11:56.851.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Benjamin Lau
SpeciesList	scientific name: Epinephelus fuscoguttatus x Epinephelus lanceolatus; NCBI TaxID: NEWT:1074816;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2026-07-31 11:40:30	ID requested
⏵ 1	2026-08-16 16:11:57	announced

Abang Zamhari DNJ, Abu Aziz N, Low CF, Lau BYC, Chong CM, Yong CSY, Lin YH, de Cruz CR, Lim LS, Yong ASK, Epinephelus lanceolatus) juveniles. Comp Biochem Physiol Part D Genomics Proteomics, 60():101965(2026) [pubmed]

10.1016/j.cbd.2026.101965;

submitter keyword: Epinephelus lanceolatus, nutrition, citric acid, label-free quantitative proteomics, liver, soybean meal, aquaculture,Hybrid grouper, Epinephelus fuscoguttatus, LC-MS/MS

Annita Yong Seok Kian
contact affiliation	Higher Institution Centre of Excellence (HICoE) Institut Marin Borneo Universiti Malaysia Sabah Jalan UMS 88450 Kota Kinabalu Sabah Malaysia
contact email	annitay@ums.edu.my
lab head
Benjamin Lau
contact affiliation	Malaysian Palm Oil Board
contact email	benjamin@mpob.gov.my
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD081994

PRIDE project URI

• PRIDE
  1. PXD081994
     1. Label: PRIDE project
     2. Name: Proteomic Insights into Hepatic Responses to High Soybean Meal Inclusion with Citric Acid Supplementation in Hybrid Grouper (Epinephelus fuscoguttatus × Epinephelus lanceolatus) Juveniles