PXD081886

PXD081886 is an original dataset announced via ProteomeXchange.

Title	WDR82 Code Coordinates Transcription and pre-mRNA 3’-End Processing Through Regulated Phosphorylation
Description	Phosphorylation plays a central role in coordinating transcription with pre-mRNA maturation, yet the mechanistic understanding of how transcription is regulated by phosphorylation remains limited. The PP1 phosphatase removes CDK9-dependent phosphorylation of Pol II and Spt5, triggering transcription termination. Here, we demonstrate that PNUTS enhances PP1 activity, whereas WDR82 mediates an interaction between PP1 and the pre-mRNA 3’-end processing machinery subunit Symplekin. We present the first structure of a PNUTS-WDR82-Symplekin-Ssu72 complex, revealing that PNUTS and Symplekin bind WDR82 through distinct interfaces using conserved short linear motifs. Mutations that inactivate PP1's catalytic activity or disrupt its interaction with either PNUTS or the 3’-end processing machinery led to impaired transcription elongation, inefficient cleavage of nascent transcripts, altered poly(A) site selection, and widespread transcription termination defects. Our findings demonstrate that the PP1 complex is essential for coupling transcription to pre-mRNA 3’-end maturation, ensuring production of functional mRNA.
HostingRepository	PRIDE
AnnounceDate	2026-07-30
AnnouncementXML	Submission_2026-07-30_04:46:18.431.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marjorie Fournier
SpeciesList	scientific name: Schizosaccharomyces pombe; NCBI TaxID: NEWT:4896;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2026-07-29 16:04:06	ID requested
⏵ 1	2026-07-30 04:46:18	announced

Dataset with its publication pending

submitter keyword: pre-mRNA processing,Transcription, phosphorylation

Lidia Vasilieva
contact affiliation	Department of Biochemistry, University of Oxford
contact email	lidia.vasilieva@bioch.ox.ac.uk
lab head
Marjorie Fournier
contact affiliation	University of Oxford
contact email	marjorie.fournier@bioch.ox.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD081886
     1. Label: PRIDE project
     2. Name: WDR82 Code Coordinates Transcription and pre-mRNA 3’-End Processing Through Regulated Phosphorylation