PXD081720

PXD081720 is an original dataset announced via ProteomeXchange.

Title	Proteomic profiling of THP1 wild‑type and ARG2‑knockout human monocytes under normoxic and hypoxic conditions
Description	Arginase 2 (ARG2) is a mitochondrial enzyme implicated in metabolic and inflammatory regulation in human monocytes. To investigate the proteomic consequences of ARG2 loss and its interaction with hypoxic stress, we performed a quantitative LC‑MS/MS analysis of THP1 wild-type (THP1WT) and ARG2‑knockout (THP1ARG2‑/‑) cells. Cells were synchronized by serum starvation and subsequently exposed for 48 hours to either normoxia (21% oxygen) or hypoxia (1% oxygen), which generates four experimental groups: THP1WT-normoxia, THP1WT‑hypoxia, THP1ARG2‑/‑‑normoxia, and THP1ARG2‑/‑‑hypoxia. Each condition was analysed in biological triplicate (12 samples total). The dataset provides a comprehensive overview of baseline proteomic differences between THP1WT and THP1ARG2‑/‑ monocytes, as well as their differential responses to hypoxic stimulation, offering a detailed resource on ARG2 related and hypoxia induced molecular changes in THP1 cells.
HostingRepository	PRIDE
AnnounceDate	2026-07-28
AnnouncementXML	Submission_2026-07-28_01:42:35.351.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michael Stumpe
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2026-07-27 09:00:31	ID requested
⏵ 1	2026-07-28 01:42:35	announced

10.1186/S12929-026-01278-3;

submitter keyword: THP1

monocytes

arginase 2

ARG2 knockout

CRISPR

hypoxia

proteomics

LC‑MS/MS

human

metabolic regulation

Dieter Kressler
contact affiliation	Department of Biology, University of Fribourg, 1700 Fribourg, Switzerland
contact email	dieter.kressler@unifr.ch
lab head
Michael Stumpe
contact affiliation	Department of Biology, University of Fribourg
contact email	michael.stumpe@unifr.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD081720

PRIDE project URI

• PRIDE
  1. PXD081720
     1. Label: PRIDE project
     2. Name: Proteomic profiling of THP1 wild‑type and ARG2‑knockout human monocytes under normoxic and hypoxic conditions