⮝ Full datasets listing
PXD081706
PXD081706 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Prohibitin Controls MRS2-Mediated Mitochondrial Mg2+ Uptake to Shape Bioenergetics |
| Description | Mitochondrial magnesium (mMg2+) is essential for cellular metabolism and bioenergetics, yet the mechanisms governing its transport remain poorly understood. Although MRS2 constitutes the pore of the mMg2+ channel, the molecular machinery regulating its function is unknown. Here, unbiased proteomics identified the prohibitin (PHB) complex as a prominent MRS2-interacting partner. Integrated biochemical and functional analyses demonstrate that the conserved coiled-coil domain mediates MRS2 homo-oligomerization, whereas the C-terminal region of MRS2 interacts with PHB1 to promote channel activity. Quantitative calibration of mitochondria-targeted MagFRET sensors revealed maximal mMg2+ uptake (appx. 15 mM), which was markedly reduced in Phb1-deficient hepatocytes. Complementary loss- and gain-of-function studies establish PHB1 as a positive regulator of MRS2-mediated mMg2+ uptake without affecting MCU-dependent Ca2+ transport. In vivo, hepatic Phb1 deletion attenuated mMg2+ uptake and enhanced cellular bioenergetics. These findings identify PHB1 as an activator of the MRS2, advancing our understanding of mMg2+ uptake machinery and its role in metabolic regulation. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-07-27 |
| AnnouncementXML | Submission_2026-07-27_07:07:30.026.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Susan T. Weintraub |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | Carbamidomethyl; Oxidation; Deamidated |
| Instrument | LTQ Orbitrap Velos Pro |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-07-27 07:00:20 | ID requested | |
| ⏵ 1 | 2026-07-27 07:07:30 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Magnesium, LC-tandem mass spectrometry, Mitochondria, MRS2, PHB1, DatasetType:Proteomics |
Contact List
| Madesh Muniswamy, M.S., Ph.D. | |
|---|---|
| contact affiliation | University of Texas Health Science Center at San Antonio |
| contact email | muniswamy@uthscsa.edu |
| lab head | |
| Susan T. Weintraub | |
| contact affiliation | UT Health Science Center San Antonio |
| contact email | weintraub@uthscsa.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v14/MSV000102618/ |
