PXD081668

PXD081668 is an original dataset announced via ProteomeXchange.

Title	Palmitoylproteomic profiles of brain tissue from wild-type and endothelial-specific PEAK1-knockout mice after cerebral ischemia/reperfusion injury
Description	To elucidate the regulatory role of protein palmitoylation in blood-brain barrier disruption during cerebral ischemia/reperfusion injury, we performed palmitoylproteomic profiling of cerebral cortical tissues from wild-type and endothelial-specific PEAK1-knockout mice subjected to middle cerebral artery occlusion/reperfusion. Site-specific mapping identified Cys1350 as the primary palmitoylation site of PEAK1 catalyzed by ZDHHC8. Pathway enrichment analysis further revealed that differentially palmitoylated proteins were mainly involved in sphingolipid signaling and ceramide biosynthesis. These findings establish ZDHHC8-mediated PEAK1 palmitoylation as a critical pathogenic regulatory node in ischemic stroke.
HostingRepository	iProX
AnnounceDate	2026-07-25
AnnouncementXML	Submission_2026-07-27_00:25:45.007.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yuanhao Xu
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2026-07-27 00:25:20	ID requested
⏵ 1	2026-07-27 00:25:45	announced

Dataset with its publication pending

submitter keyword: S-palmitoylation, Palmitoylproteomics, Ischemic stroke, Blood-brain barrier, PEAK1, ZDHHC8

Junping Kou
contact affiliation	China Pharmaceutical University
contact email	junpingkou@cpu.edu.cn
lab head
Yuanhao Xu
contact affiliation	China Pharmaceutical University
contact email	17826065610@163.com
dataset submitter

iProX dataset URI