PXD081593

PXD081593 is an original dataset announced via ProteomeXchange.

Title	Quantitative Glycoproteomic Profiling Identifies Distinct N‑glycosylation Signatures in Renal Biopsies from Healthy Kidney Donors and Diabetic Kidney Disease Patients
Description	Diabetic kidney disease (DKD) is the leading cause of end-stage renal disease (ESRD) worldwide, but site-specific N-glycosylation signatures underlying DKD renal damage remain poorly characterized. Unlike previous studies relying on suboptimal control tissues, we utilized intact healthy kidney donor (HKD) cortical biopsies as physiologically accurate references. We applied an optimized quantitative glycoproteomic pipeline combining homogenization and non-contact ultrasonic-assisted protein extraction, ZIC-HILIC enrichment, and EThcD-sceHCD-MS/MS to profile intact N-glycopeptides (IGPs) from 20 DKD patients and 20 HKD controls. We identified 1,186 significant differentially expressed IGPs and 111 altered N-glycans derived from 294 glycoproteins in DKD renal tissues (adjusted p < 0.05, FC > 1.5 or < 0.67).
HostingRepository	iProX
AnnounceDate	2026-07-24
AnnouncementXML	Submission_2026-07-24_02:08:23.904.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yong Zhang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	deaminated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2026-07-24 02:07:37	ID requested
⏵ 1	2026-07-24 02:08:24	announced

Dataset with its publication pending

submitter keyword: renal tissue, healthy kidney donor, diabetic kidney disease, N-glycosylation, glycoproteomics.

Yong Zhang
contact affiliation	West China Hospital, Sichuan University
contact email	nankai1989@foxmail.com
lab head
Yong Zhang
contact affiliation	West China Hospital, Sichuan University
contact email	nankai1989@foxmail.com
dataset submitter

iProX dataset URI