⮝ Full datasets listing
PXD081583
PXD081583 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | BLTP2 bridges the ER and MVB for exosome biogenesis at SCAMP3-dependent ER contacts |
| Description | Multivesicular bodies (MVBs) contain intraluminal vesicles (ILVs) designated for degradation in lysosomes or release as exosomes for cell-to-cell communication. The mechanisms governing ILV/exosome formation are not fully understood. Here, we show that the integral endoplasmic reticulum (ER) membrane protein bridge-like lipid transfer protein 2 (BLTP2; KIAA0100) is indispensable in ILV/exosome formation and that secretory carrier membrane protein 3 (SCAMP3) recruits BLTP2 to ER–MVB membrane contact sites (MCSs) in a Rab5-dependent manner. Our results indicate that this recruitment is hindered by NEDD4-mediated ubiquitination of SCAMP3. Depletion of BLTP2 was found to impede ILV/exosome formation and selectively reduced the levels of cone-shaped phospholipids, including bis(monoacylglycero)phosphate (BMP), and the BMP precursor phosphatidylglycerol (PG) within endosomes. BLTP2 knockout also hampered cell proliferation and tumorigenicity, which could be restored by supplementation with exosomes from wild-type cells. Our findings suggest that BLTP2 transfers the BMP/LBPA precursor PG to MVBs for BMP/LBPA synthesis and promotes ILV/exosome formation at SCAMP3-dependent ER–MVB MCSs. |
| HostingRepository | iProX |
| AnnounceDate | 2026-07-23 |
| AnnouncementXML | Submission_2026-07-24_01:50:02.277.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Dongchen Li |
| SpeciesList | scientific name: Mus musculus; NCBI TaxID: 10090; scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | acetylated residue; methionine oxidation with neutral loss of 64 Da |
| Instrument | Orbitrap Fusion Lumos; Orbitrap Astral |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-07-24 01:49:11 | ID requested | |
| ⏵ 1 | 2026-07-24 01:50:02 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: BLTP2 |
Contact List
| Weike Ji | |
|---|---|
| contact affiliation | Huazhong University of Science and Technology |
| contact email | Weike_Ji1985@163.com |
| lab head | |
| Dongchen Li | |
| contact affiliation | Huazhong University of Science and Technology |
| contact email | 1310431117@qq.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
