PXD081428

PXD081428 is an original dataset announced via ProteomeXchange.

Title	The absence of functional CAF-1 results in deficiencies in H3.3 levels at transcribed loci in Arabidopsis
Description	Eukaryotic organisms organize their genetic material in chromatin rather than naked DNA. Efficient chromatin assembly is essential for all DNA-templated processes, including replication, transcription, and DNA repair. Our research focused on two major histone H3 deposition complexes: Chromatin Assembly Factor (CAF-1), which deposits canonical H3 on naked DNA immediately after replication during the S-phase in yeast and metazoans, and the Histone Regulator A (HIRA) complex, which is thought to incorporate the H3.3 variant in a replication-independent manner. By combining biochemical, genetic, genomic, and proteomic data, we revealed that CAF-1 activity might be associated with the maintenance of the histone variant H3.3 in Arabidopsis chromatin. Moreover, this CAF-1's potential function in H3.3 homeostasis may be particularly significant in the transcribed regions of the genome.
HostingRepository	iProX
AnnounceDate	2026-07-21
AnnouncementXML	Submission_2026-07-21_19:31:55.951.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Fan Yang
SpeciesList	scientific name: Arabidopsis thaliana; NCBI TaxID: 3702;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 240; Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2026-07-21 19:31:38	ID requested
⏵ 1	2026-07-21 19:31:56	announced

Dataset with its publication pending

submitter keyword: Epigenetics, Chromatin, Histone Variants, Histone Chaperones,Transcription

Israel Ausin
contact affiliation	Northwest A&F University
contact email	israel.ausin@gmail.com
lab head
Fan Yang
contact affiliation	Northwest A&F University
contact email	547533956@qq.com
dataset submitter

iProX dataset URI