⮝ Full datasets listing
PXD081338
PXD081338 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Systematic proteomics identifies a conserved mechanism for polar targeting of plant cortical proteins |
| Description | Multicellular development is tightly coupled to the polarization of individual cells, which partitions polar proteins along the cell cortex and can drive asymmetric cell division, anisotropic growth, local differentiation or physiology. Mechanisms driving cell polarization have been described in fungi and animals, but these lack counterparts in plants. While several polar proteins have been identified in plants, the mechanisms guiding their polar localization are largely elusive. Through iterative affinity proteomics on the recently identified SOSEKI polar proteins, we identify a network of polar proteins that is largely conserved in the flowering plant Arabidopsis and the liverwort Marchantia. We next used this collection of novel polar proteins for systematic proximity ligation proteomics in these two species to map their global polar proteome. We identify a subfamily of polarized Protein Acyltransferase (PAT) enzymes that are required for membrane targeting of Arabidopsis SOSEKI proteins. Using human and fruit fly models, we show that PAT19 is sufficient for membrane targeting of SOSEKI1, likely through direct palmitoylation. This work offers a rich resource for studying polar protein localization, and identifies a conserved mechanism for polar protein targeting in plants. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-07-20 |
| AnnouncementXML | Submission_2026-07-20_08:37:08.794.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Mark Roosjen |
| SpeciesList | scientific name: Arabidopsis thaliana; common name: thale cress; NCBI TaxID: 3702; scientific name: Marchantia polymorpha; common name: liverwort; NCBI TaxID: 3197; |
| ModificationList | Carbamidomethyl |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-07-20 06:23:01 | ID requested | |
| ⏵ 1 | 2026-07-20 08:37:09 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Proxitome, polarity, TurboID, SOSEKI, DatasetType:Proteomics |
Contact List
| Dolf Weijers | |
|---|---|
| contact affiliation | Wageningen University and Research |
| contact email | dolf.weijers@wur.nl |
| lab head | |
| Mark Roosjen | |
| contact affiliation | Wageningen University and Research |
| contact email | mark.roosjen@wur.nl |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v13/MSV000102532/ |
