PXD081253

PXD081253 is an original dataset announced via ProteomeXchange.

Title	Proteomics of Cellular Vesicles Derived from Cells Treated with Different Drugs
Description	To investigate the molecular mechanisms by which the combination of doxorubicin and apatinib induces enhanced immunogenic cell death (ICD) in tumor cells, and to identify the proteins carried by the resulting nanovesicles, we conducted a comprehensive proteomic analysis. Mouse triple-negative breast cancer 4T1 cells were exposed to the following three conditions: (1) untreated (control group); (2) treated with doxorubicin alone (10 μM, 24 hours); (3) Co-treated with doxorubicin (10 μM) and apatinib (10 μM) for 24 hours to induce chemotherapy-sensitized ICD. After treatment, cells were harvested and sequentially extruded through polycarbonate membranes with pore sizes of 10, 5, 1, and 0.1 μm to prepare nanovesicles. The resulting vesicle populations were designated as BEVs (designated as “N” in the raw data), Dox-BEVs (designated as “D” in the raw data), and VICIs (designated as “D-A” in the raw data), and were subjected to in-solution digestion. Subsequently, the resulting peptide mixtures were analyzed by liquid chromatography–tandem mass spectrometry (LC-MS/MS).
HostingRepository	PRIDE
AnnounceDate	2026-09-03
AnnouncementXML	Submission_2026-09-03_01:57:53.323.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yao Xuxiang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	acetylated residue
Instrument	LTQ

Revision	Datetime	Status	ChangeLog Entry
0	2026-07-17 07:35:09	ID requested
⏵ 1	2026-09-03 01:57:53	announced

Dataset with its publication pending

submitter keyword: Apatinib,VICIs,Dox,BEVs

Xuxiang Yao1,6, Chengyi Hu1,6, Kejian Ding1,6, Pan Fu1, Lin Li1, Sihua Qian1, Yuhui Wang1, Akinori Kuzuya4, Jianping Zheng5,1,*, Chunhai Fan3, Jiang Li2,*, Kaizhe Wang1,*
contact affiliation	1 Ningbo Key Laboratory of Biomedical Imaging Probe Materials and Technology, Ningbo Cixi Institute of Biomedical Engineering, Ningbo Institute of Materials Technology and Engineering of Chinese Academy of Sciences, Ningbo, 315300, China.
contact email	wangkaizhe@nimte.ac.cn
lab head
Yao Xuxiang
contact affiliation	Ningbo Cixi Institute of Biomedical Engineering
contact email	yaoxuxiang@tju.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD081253

PRIDE project URI

• PRIDE
  1. PXD081253
     1. Label: PRIDE project
     2. Name: Proteomics of Cellular Vesicles Derived from Cells Treated with Different Drugs