PXD081233

PXD081233 is an original dataset announced via ProteomeXchange.

Title	Sacral Neural Crest-Derived Extracellular Vesicles Suppress Pyroptosis via the ANXA1-FPR2 Axis to Repair Pelvic Ganglia
Description	Pelvic autonomic nerve injury is a major cause of postoperative bowel dysfunction, yet effective strategies for restoring damaged pelvic ganglia remain lacking. Here, we demonstrate that extracellular vesicles derived from human pluripotent stem cell–induced sacral neural crest cells (Sacral NC-EVs) promote pelvic neuroregeneration by suppressing oxidative stress–induced pyroptosis. In a rat model of major pelvic ganglion (MPG) injury, local administration of Sacral NC-EVs improved gastrointestinal motility and partially restored rectal neural innervation and epithelial barrier integrity. Mechanistically, MPG injury induced a hypoxia-associated oxidative microenvironment that activated the NLRP3–caspase-1–GSDMD pyroptosis pathway, whereas Sacral NC-EVs effectively attenuated oxidative stress and inhibited pyroptotic signaling. Comparative analyses showed that Sacral NC-EVs produced more pronounced overall therapeutic effects than mesenchymal stem cell–derived and vagal neural crest–derived EVs, highlighting a functional advantage of developmental lineage matching. Proteomic and functional studies identified annexin A1 (ANXA1) as an important EV-associated mediator that acts through FPR2 signaling to contribute to the antioxidant and anti-pyroptotic effects of Sacral NC-EVs. Collectively, our findings establish lineage-matched EVs as a mechanism-driven, cell-free strategy for neural repair by targeting the oxidative stress–pyroptosis axis.
HostingRepository	iProX
AnnounceDate	2026-07-17
AnnouncementXML	Submission_2026-07-16_20:40:47.349.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ting Yang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2026-07-16 20:38:48	ID requested
⏵ 1	2026-07-16 20:40:47	announced

Dataset with its publication pending

submitter keyword: Sacral neural crest cells, Major pelvic ganglia, Pyroptosis, Extracellular vesicles, Colorectal dysfunction

Wu Song
contact affiliation	Department of Gastrointestinal Surgery, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, 510080, China.
contact email	songwu@mail.sysu.edu.cn
lab head
Ting Yang
contact affiliation	Department of Gastrointestinal Surgery, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, 510080, China.
contact email	1501592357@qq.com
dataset submitter

iProX dataset URI