⮝ Full datasets listing
PXD081217
PXD081217 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Global, site-resolved mapping of endogenous lipid modifications reveals an extensive S-acylation landscape of ribosomes -AirID data- |
| Description | Emerging evidence indicates that ribosomes are regulated by various post-translational modifications to maintain proteostasis. However, the global landscape and functional impacts of lipid modification on ribosomes remain largely unexplored, due to technical limitations. Here, we present a simple, probe- and chemical-reaction-free workflow that enables global, site-resolved analysis of intact protein lipidation. Using this approach, we identify over 1,200 endogenous lipidation sites and simultaneously detect multiple lipid modifications, including myristoylation, palmitoylation, and prenylation, revealing hundreds of previously unreported lipidation events on diverse proteins. Applying this method to human ribosomes reveals an extensive landscape of long-chain S-acylation, with approximately 75% of cysteine-containing ribosomal proteins modified. Functional analysis shows that palmitoylation at cysteine 66 of RPS5/uS7 influences nuclear export of pre-40S ribosomes. These findings suggest a regulatory role for S-acylation in ribosome biology, offering new insights into the modulation of translation machinery through lipid modifications. We performed AirID-based proximitiy labeling proteomics. |
| HostingRepository | jPOST |
| AnnounceDate | 2026-09-01 |
| AnnouncementXML | Submission_2026-08-31_08:00:04.948.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Koshi Imami |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
| ModificationList | S-carboxamidomethyl-L-cysteine; unknown modification |
| Instrument | Q Exactive Plus; Orbitrap Eclipse |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-07-16 16:26:47 | ID requested | |
| ⏵ 1 | 2026-08-31 08:00:05 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Lipidation, ribosome, AirID, proximity labeling |
Contact List
| Koshi Imami | |
|---|---|
| lab head | |
| Koshi Imami | |
| contact affiliation | RIKEN |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST004770/ |
