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PXD081153
PXD081153 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Comparative Proteomic Profiling of Extracellular Vesicles from Four Liver Cell Lines Isolated by PHS Capture versus Ultracentrifugation |
| Description | This study aims to compare the proteomic profiles of extracellular vesicles (EVs) isolated from four human liver cell lines—THLE-2 (non-tumorigenic immortalized hepatocytes), HepG2, HEP3B, and Huh7 (hepatocellular carcinoma cell lines)—using two different isolation methods: PHS capture and ultracentrifugation (UC). The goal is to identify method-dependent and cell line-specific protein signatures in EVs, with particular focus on tumor-associated biomarkers. Mass spectrometry-based proteomic analysis was performed to quantify protein expression levels across all samples. Differential expression analysis revealed distinct protein profiles between the four cell lines, as well as differences attributable to the isolation methods employed. Key tumor-associated biomarkers are highlighted in the dataset for further investigation. This dataset provides a valuable resource for understanding EV protein cargo heterogeneity in liver cancer and for evaluating the performance of PHS capture as an alternative EV isolation technique compared to the conventional UC method. The raw mass spectrometry data and protein identification results have been submitted to iProX in accordance with ProteomeXchange data sharing policies. |
| HostingRepository | iProX |
| AnnounceDate | 2026-07-15 |
| AnnouncementXML | Submission_2026-08-31_20:17:49.215.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Xifeng Mo |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Astral |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-07-15 18:58:54 | ID requested | |
| ⏵ 1 | 2026-08-31 20:17:49 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Extracellular vesicles, Exosomes, Liver cancer, Hepatocellular carcinoma, THLE-2, HepG2, HEP3B, Huh7, Proteomics, Differential protein expression, PHS capture, Ultracentrifugation, Tumor biomarkers, Cell line comparison, Mass spectrometry |
Contact List
| Fan Yang | |
|---|---|
| contact affiliation | Guangxi Medical University |
| contact email | yangfan@gxmu.edu.cn |
| lab head | |
| Xifeng Mo | |
| contact affiliation | Guangxi Medical University |
| contact email | 2232482616@qq.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
