⮝ Full datasets listing
PXD081081
PXD081081 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | The ER membrane protein complex acts as a chaperone to promote the biogenesis of multi-bundle membrane proteins |
| Description | Nearly half of the ~5,000 human membrane proteins need to assemble into stoichiometric complexes as part of their biogenesis at the endoplasmic reticulum (ER) membrane. How ER resident biogenesis factors coordinate membrane insertion, folding and assembly is not well understood. Here, we demonstrate that the ER membrane protein complex (EMC) insertase additionally acts as a chaperone to facilitate the assembly of heterotrimeric voltage-gated calcium channels (Cav). Using function-separating mutations and inhibitory nanobodies we show that nascent Cav channels are degraded prematurely when EMC's chaperone function is selectively perturbed. Blocking EMC's chaperone function strongly impaired Cav-dependent cardiomyocyte contraction. EMC engagement of the pore-forming Cav alpha-subunit occurred co-translationally and required Cav alpha's first transmembrane domain bundle to protrude from the nascent ribosome Sec61 multipass translocon complex. Our findings establish a chaperone function for the EMC and reveal that biogenesis of multi-bundle membrane proteins requires a highly orchestrated, co-translational interplay between ER biogenesis factors. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-08-28 |
| AnnouncementXML | Submission_2026-08-28_13:56:41.644.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Dain Brademan |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | Carbamidomethyl; Oxidation |
| Instrument | timsTOF HT |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-07-14 15:43:08 | ID requested | |
| ⏵ 1 | 2026-08-28 13:56:42 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: timsTOF, dia-PASEF, bottom-up proteomics, DatasetType:Proteomics |
Contact List
| Ruth Huttenhain | |
|---|---|
| contact affiliation | Department of Molecular and Cellular Pharmacology, UCSF |
| contact email | Ruth.huttenhain@ucsf.edu |
| lab head | |
| Dain Brademan | |
| contact affiliation | Stanford School of Medicine |
| contact email | brademan@stanford.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v13/MSV000102484/ |
