⮝ Full datasets listing
PXD080956
PXD080956 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Distribution, assembly and mechanism of GluN1/GluN3A excitatory glycine receptors |
| Description | NMDA receptors play key roles in brain development, plasticity and diseases. While glutamate and glycine co-gated GluN2-containing NMDARs have been extensively characterized, little is known regarding GluN3A-containing NMDARs that form receptors gated by glycine only. Here, combining native purification, mass spectrometry, cryo-EM and electrophysiology, we provide key insights on the molecular logic of GluN3A-NMDARs. We demonstrate that native GluN3A receptors account for a sizeable fraction of total NMDARs, are enriched at extrasynaptic compartments in the adult brain, and assemble as diheteromeric GluN1/GluN3A excitatory glycine receptors (eGlyRs) rather than as triheteromeric GluN1/GluN2/GluN3A receptors. The architecture of eGlyRs reveal splayed and loosely packed extracellular domains, strikingly different from ‘conventional’ GluN1/GluN2 receptors. Through back-and-forth structural and functional validations, we demonstrate how the combined effects of a weak ligand-binding domain (LBD) dimer interface and high intrinsic mobility of the N-terminal domains (NTDs) shape the atypical gating of eGlyRs. These findings illuminate GluN3A-NMDAR physiology and mechanism, with implications for neuronal signaling and pharmacology. |
| HostingRepository | iProX |
| AnnounceDate | 2026-07-13 |
| AnnouncementXML | Submission_2026-07-13_01:43:27.412.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Nan Zhao |
| SpeciesList | scientific name: Mus musculus; NCBI TaxID: 10090; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-07-13 01:43:11 | ID requested | |
| ⏵ 1 | 2026-07-13 01:43:28 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: NMDAR, GluN1/GluN3A, CryoEM, Mass Spectrometry, electrophysiology |
Contact List
| Lihua Zhang | |
|---|---|
| contact affiliation | Dalian Institute of Chemical Physics |
| contact email | lihuazhang@dicp.ac.cn |
| lab head | |
| Nan Zhao | |
| contact affiliation | Dalian Institute of Chemical Physics |
| contact email | zhaonan@dicp.ac.cn |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
