PXD080931

PXD080931 is an original dataset announced via ProteomeXchange.

Title	DDX19B as a nuclear-pore-associated regulator of the post-meiotic 3′UTR landscape
Description	Purified early spermatids (approximately 5×106 cells) from HA-tagged Ddx19b KI and WT control mice were lysed in IP lysis buffer (50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1% NP-40, and protease inhibitors). Lysates were clarified by centrifugation and incubated with anti-HA magnetic beads overnight at 4°C. Beads were washed extensively, and bound proteins were eluted with 0.1 M glycine-HCl pH 2.5. Eluted proteins were neutralized, reduced, alkylated, and digested with trypsin. The resulting peptides were analyzed by nano-LC-MS/MS on an Orbitrap Fusion Lumos mass spectrometer. Peptide identification and label-free quantification were performed using MaxQuant software against the UniProt mouse database. The protein intensitiesof all identified proteins were normalized bytotal intensity and then used for differentialprotein expression analysis using DEqMS(Zhu et al. 2020). Proteins with a log2FC > 2 and an FDR < 0.01 were considered significant interactors.
HostingRepository	iProX
AnnounceDate	2026-07-10
AnnouncementXML	Submission_2026-07-13_01:11:53.637.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xinghai Yu
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2026-07-13 01:11:34	ID requested
⏵ 1	2026-07-13 01:11:54	announced

Dataset with its publication pending

submitter keyword: DDX19B, mRNA export

Junyan Kang
contact affiliation	Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine
contact email	kangjunyan@outlook.com
lab head
Xinghai Yu
contact affiliation	Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine
contact email	yuxinghai@sjtu.edu.cn
dataset submitter

iProX dataset URI