PXD080923

PXD080923 is an original dataset announced via ProteomeXchange.

Title	High-titer L-lactic acid production from corn stover using Pediococcus acidilactici
Description	L-Lactic acid is a key monomer for polylactic acid (PLA), and its market demand continues to grow. Producing lactic acid from lignocellulosic feedstocks instead of grain can alleviate competition for food resources; however, inhibitors generated during pretreatment result in low fermentation titers and complex downstream processes, hindering industrial implementation. In this study, Pediococcus acidilactici NHRI04 with robust inhibitor tolerance was obtained through staged adaptive domestication, enabling direct fermentation of undetoxified corn stover hydrolysate. Process optimization achieved a final L-lactic acid concentration of 226.09 g/L, with a productivity of 3.12 g/L/h and a sugar-to-lactic acid yield of 99.72%, corresponding to an overall yield of 0.32 g/g corn stover and 98.80% optical purity. This titer represents the highest lactic acid concentration reported to date from lignocellulosic biomass. A pasteurization-based fermentation strategy (65 °C, 30 min) was established to replace conventional autoclaving, effectively preserving medium nutritional value. Economic analysis revealed that combining high-concentration fermentation, pasteurization, and detoxification elimination reduced production costs by 2,232 CNY per ton of lactic acid. Substituting corn stover for corn grain saves 1.8 tons of corn and reduces carbon emissions by 1.83 tons CO₂e per ton of lactic acid. Integrated whole-genome, transcriptomic, and proteomic analyses elucidated the molecular mechanisms underlying inhibitor tolerance. This study provides a feasible approach for low-cost, grain-saving, and low-carbon production of lignocellulosic lactic acid.
HostingRepository	iProX
AnnounceDate	2026-07-09
AnnouncementXML	Submission_2026-07-13_01:03:02.054.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ao Zhang
SpeciesList	scientific name: Pediococcus acidilactici; NCBI TaxID: 1254;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2026-07-13 01:02:23	ID requested
⏵ 1	2026-07-13 01:03:02	announced

Dataset with its publication pending

submitter keyword: Pediococcus acidilactici, L-lactic acid, lignocellulose, adaptive evolution

Peiyong Qin
contact affiliation	Beijing University of Chemical Technology
contact email	2005500090@buct.edu.cn
lab head
Ao Zhang
contact affiliation	Beijing University of Chemical Technology
contact email	2090695919@qq.com
dataset submitter

iProX dataset URI