PXD080813

PXD080813 is an original dataset announced via ProteomeXchange.

Title	Functional Mapping of the Trypanosoma cruzi Serinome by Fluorophosphonate Activity-Based Protein Profiling
Description	Serine hydrolases (SHs) constitute one of the largest enzyme superfamilies in eukaryotes, yet their roles in Trypanosoma cruzi, the causative agent of Chagas disease, remain largely uncharacterized. Here, we report an activity-based chemoproteomic map of the T. cruzi epimastigote serinome by combining genome-informed in silico curation with whole-cell activity-based protein profiling (ABPP) using a panel of cell-permeable fluorophosphonate (FP)-alkyne probes. Whole-cell labelling followed by label-free quantitative proteomics (LFQ-MS), identified 37 enriched SH-like proteins, including 35 with conserved or partially conserved catalytic triad/dyad features, spanning lipases, peptidases, esterases, and previously uncharacterized hydrolases. The 35 SHs represent approximately 63 % of the 56 predicted SHs retained after catalytic-site curation. Domain architecture analysis revealed broad structural diversity, while orthologue-based localization data suggested association with multiple subcellular compartments, including glycosomal, mitochondrial, and endosomal localizations. Gene Ontology enrichment highlighted lipid metabolic and catabolic processes as dominant functional themes, and protein–protein interaction network analysis supported functional connectivity among the captured enzymes. Several identified SHs, including oligopeptidase B, prolyl oligopeptidase Tc80, serine carboxypeptidase CPB1, and phospholipase A1 (PLA1) have previously been characterized in trypanosomatids as virulence factors and as mediator of host-pathogen interactions. Together, these findings establish a fluorophosphonate-based chemoproteomic resource for the kinetoplastid community and prioritize probe-accessible active T. cruzi SHs for future functional validation and antiparasitic inhibitor discovery.
HostingRepository	PRIDE
AnnounceDate	2026-07-21
AnnouncementXML	Submission_2026-07-21_02:59:02.480.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jaime Ariel Isern
SpeciesList	scientific name: Trypanosoma cruzi Dm28c; NCBI TaxID: NEWT:1416333;
ModificationList	acetylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2026-07-09 11:52:53	ID requested
⏵ 1	2026-07-21 02:59:03	announced

10.64898/2026.07.18.739127;

submitter keyword: Activity-based protein profiling, Chagas disease, Fluorophosphonate probes, Serine hydrolases,Trypanosoma cruzi

Patrick G. Steel
contact affiliation	Department of Chemistry, Durham University, Stockton Rd, DH1 3LE, Durham, United Kingdom
contact email	p.g.steel@durham.ac.uk
lab head
Jaime Ariel Isern
contact affiliation	Durham University
contact email	jaime.a.isern@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD080813
     1. Label: PRIDE project
     2. Name: Functional Mapping of the Trypanosoma cruzi Serinome by Fluorophosphonate Activity-Based Protein Profiling