PXD080791

PXD080791 is an original dataset announced via ProteomeXchange.

Title	Screening and Bioinformatics Analysis of Differential Proteins in Thiram-Induced Tibial Dyschondroplasia of Daheng Broilers Based on Astral DIA Proteomics
Description	This study aimed to screen differentially expressed proteins (DEPs) in the tibial growth plate of thiram-induced tibial dyschondroplasia (TD) in Daheng broilers and to conduct bioinformatics analysis, providing potential targets for exploring the molecular mechanism of TD.One-day-old Daheng broilers were randomly divided into control group (NC) and TD model group (fed with 100 mg/kg thiram). Tibial cartilage growth plate tissues were collected at 17 days of age, and HE staining was performed to confirm pathological changes. Three biological replicates per group were subjected to Astral DIA label-free quantitative proteomic sequencing, and DEPs were screened with |log₂FC| ≥ 1 and P < 0.05. Principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA), GO annotation, KEGG pathway enrichment analysis, GSEA, and protein-protein interaction (PPI) network were performed to screen core candidate proteins. HE staining showed that the TD group exhibited significantly thickened tibial growth plate, disorganized chondrocyte arrangement, and typical cartilage plug formation, indicating successful TD model establishment. A total of 7 561 proteins were identified by proteomics, with 7 300 quantifiable proteins. PCA and PLS-DA analysis showed good separation between the two groups. A total of 297 DEPs were screened, including 131 up-regulated and 166 down-regulated proteins. GO analysis indicated that DEPs were mainly enriched in biological processes such as negative regulation of ossification, regulation of Notch signaling pathway, reactive oxygen species metabolism, and regulation of mitochondrial membrane potential; cellular components were mainly localized in the extracellular matrix; molecular functions mainly involved methyltransferase activity and heparin binding. KEGG significantly enriched pathways included ECM-receptor interaction, glycosaminoglycan biosynthesis, and focal adhesion. GSEA further validated the enrichment of pathways such as phagosome and phosphatidylinositol signaling system. PPI network analysis screened out core node proteins including BIRC3, BCL2, MRPS14, ASPN, POSTN, COL5A1, and DAP3. This study revealed the differential protein expression profile of thiram-induced TD in Daheng broilers at the protein level. The screened candidate proteins may be closely related to abnormal cartilage development, providing new clues for in-depth understanding of the pathogenesis of TD.
HostingRepository	iProX
AnnounceDate	2026-07-09
AnnouncementXML	Submission_2026-07-09_02:10:57.242.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shiliang Zhu
SpeciesList	scientific name: Gallus gallus; NCBI TaxID: 9031;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2026-07-09 02:10:37	ID requested
⏵ 1	2026-07-09 02:10:57	announced

Dataset with its publication pending

submitter keyword: Daheng broiler, Tibial dyschondroplasia, Thiram, DIA proteomics, Gallus gallus

Chaowu Yang
contact affiliation	Sichuan Academy of Animal Sciences
contact email	cwyang@foxmail.com
lab head
Shiliang Zhu
contact affiliation	Sichuan Academy of Animal Sciences
contact email	981728743@qq.com
dataset submitter

iProX dataset URI