PXD080709

PXD080709 is an original dataset announced via ProteomeXchange.

Title	Acetylome profiling of glioblastoma cells in response to rapamycin treatment
Description	We employed the human glioblastoma cell line LN229 as a model system. Cells were treated with rapamycin (200 nM, 12 h) to induce autophagic stress, and acetylproteomic profiling was performed using high resolution LC MS/MS combined with immunoaffinity enrichment of acetylated peptides. We quantified 450 acetylated peptides corresponding to 333 proteins after normalization analysis when we applied a cut-off peptide abundance of 1.2-fold (log2). Interestingly, the acetylation of the majority of proteins, 439 peptides from 324 proteins, decreased after rapamycin treatment. Our dataset provides a comprehensive resource for understanding how acetylation integrates metabolic and epigenetic signals to sustain glioblastoma cell fitness under autophagic pressure. The findings may offer novel targets for combinatorial therapies that disrupt this adaptive acetylation network.
HostingRepository	iProX
AnnounceDate	2026-07-07
AnnouncementXML	Submission_2026-07-08_00:09:30.325.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yuntao Lu
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	acetylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2026-07-08 00:03:15	ID requested
⏵ 1	2026-07-08 00:09:30	announced

Dataset with its publication pending

submitter keyword: acetylomics

Yuntao Lu
contact affiliation	Nanfang Hospital, Southern Medical University
contact email	lllu2000yun@gmail.com
lab head
Yuntao Lu
contact affiliation	Nanfang Hospital, Southern Medical University
contact email	lllu2000yun@gmail.com
dataset submitter

iProX dataset URI