PXD080682

PXD080682 is an original dataset announced via ProteomeXchange.

Title	Crosslinking mass spectrometry of the α/β subunit interface in urinary and recombinant human chorionic gonadotropin (hCG)
Description	Human chorionic gonadotropin (hCG) is a heterodimeric glycoprotein hormone whose urinary (u-hCG) and recombinant (r-hCG) forms differ in glycosylation and stability. To test whether this reflects a structural reorganization of the α/β subunit interface, the two forms were compared by chemical crosslinking mass spectrometry (XL-MS). Crosslinking was performed on the intact, natively glycosylated heterodimers using two complementary chemistries: the lysine-reactive reagent DSS and the zero-length coupling reagent DMTMM (targeting Lys-Asp/Glu pairs, each in biological triplicate for both forms. Identified inter-subunit crosslinks were mapped onto the hCG structure to evaluate whether the assembled core interface is conserved between u-hCG and r-hCG. The dataset comprises the raw LC-MS/MS files and the corresponding crosslink identifications for all conditions and replicates, and accompanies a broader native-MS, ion-mobility and collision-induced-unfolding study of the two hCG forms.
HostingRepository	PRIDE
AnnounceDate	2026-07-15
AnnouncementXML	Submission_2026-07-15_02:43:01.939.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dinko Soic
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2026-07-07 07:45:56	ID requested
⏵ 1	2026-07-15 02:43:02	announced

Dataset with its publication pending

submitter keyword: subunit interface,human chorionic gonadotropin, crosslinking mass spectrometry, structural proteomics

Alexander Leitner
contact affiliation	ETH Zurich, Institute of Molecular Systems Biology, Zurich, Switzerland
contact email	leitner@imsb.biol.ethz.ch
lab head
Dinko Soic
contact affiliation	ETH Zürich
contact email	soic@imsb.biol.ethz.ch
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD080682
     1. Label: PRIDE project
     2. Name: Crosslinking mass spectrometry of the α/β subunit interface in urinary and recombinant human chorionic gonadotropin (hCG)