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PXD080679
PXD080679 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Phosphorylation of the AP2 μ2 subunit by p70S6 kinase facilitates clathrin-mediated endocytosis. |
| Description | Clathrin-mediated endocytosis (CME) internalizes cell-surface receptors via clathrin-coated invaginations of the plasma membrane. Both clathrin and endocytic cargo are recruited to these sites by the adaptor protein complex AP2. AP2 cycles between a closed cytoplasmic conformation and an open membrane-bound state, and efficient CME requires both conformations and their dynamic interconversion. The mechanisms regulating these conformational changes, which include post-translational modifications of the AP2, remain incompletely understood. Here, we report that p70S6 kinase phosphorylates the µ2 subunit of the AP2 and that the phosphorylation of serine 45 (S45) depends on p70S6K activity. Loss of S45-µ2 phosphorylation results in decreased internalization of canonical CME cargo such as transferrin and PDGF receptors. In Caenorhabditis elegans, lack of S45-µ2 phosphorylation produces directionally similar but markedly weaker phenotypes than AP2 loss of function. Live imaging and in silico dynamic modelling suggest that S45-μ2 phosphorylation has impact on the conformational changes of the AP2 complex. These findings identify a p70S6K-dependent mechanism that modulates AP2 function and highlight the importance of post-translational regulation in controlling CME. |
| HostingRepository | jPOST |
| AnnounceDate | 2026-07-31 |
| AnnouncementXML | Submission_2026-08-21_04:27:28.471.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Mass Spectrometry Laboratory IBB PAS |
| SpeciesList | scientific name: Rattus rattus (Black rat); NCBI TaxID: 10117; |
| ModificationList | S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine |
| Instrument | LTQ Orbitrap Elite |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-07-07 07:33:07 | ID requested | |
| ⏵ 1 | 2026-08-21 04:27:29 | announced |
Publication List
| Tempes A, Brzozowska A, W, ę, gierski T, Fasemire A, Olek K, Jastrz, ę, bski K, Liszewska E, Misztal K, Machnicka K, Macias M, Szybi, ń, ska A, Sitkiewicz E, Malinowska A, Gozdz A, Wyszy, ń, ska A, Ł, asica A, Hoffmann-M, ł, odzianowska M, Orzo, ł K, Mi, ą, czy, ń, ska M, G, ó, rna MW, Pokrzywa W, Jaworski J, Malik AR, 2 subunit by p70S6 kinase facilitates clathrin-mediated endocytosis. J Cell Sci, 139(17):(2026) [pubmed] |
Keyword List
| submitter keyword: AP2, phosphorylation, p70S6 kinase |
Contact List
| Anna R Malik | |
|---|---|
| lab head | |
| Mass Spectrometry Laboratory IBB PAS | |
| contact affiliation | Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, PL |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST004745/ |
