PXD080664

PXD080664 is an original dataset announced via ProteomeXchange.

Title	Harnessing BODIPY Carbocation Intermediate for Rapid Protein Proximity Modification
Description	The attractive photo-decaging chemistries of boron-dipyrromethene (BODIPY) chromophores have been well-established with diverse photochemical applications. This work, however, discovers that meso-substituted BODIPY chromophores can modify proteins in proximity via photo-decaging into hyper-reactive carbocation. Structural modulations of BODIPY photocages accelerate protein labeling kinetics up to 40-fold with t1/2 = 34.6 s. The BODIPY photocage preferentially labels cysteine, lysine and other nucleophilic amino acids via carbocation but not radical intermediate. At low concentration, the BODIPY-mediated labeling reaction only occurs in proximity to proteins due to aqueous quenching of the hyper-reactive carbocation intermediate, resulting in spatially-confined labeling selectivity. When grafted to mitochondria-guiding triphenylphosphine, the BODIPY-PPh3 probe enables stable covalent imaging of depolarized mitochondria, which lose membrane potential to absorb classical non-covalent mitochondria tracers. Harnessing its inherent lipid droplet (LD)-targeting property, the BODIPY-LD probe with an alkyne enrichment handle captures and profiles the dynamic LD interacting proteome in living cells, revealing RTN4 as a LD regulator. Together, the previously overlooked carbocation intermediates of fluorescent photocages (BODIPY, coumarin & cyanine etc.) may expand the toolbox of proximity labeling technologies for spatiotemporal imaging and proteomic analyses.
HostingRepository	iProX
AnnounceDate	2026-07-07
AnnouncementXML	Submission_2026-07-07_01:34:26.498.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nan Zhao
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2026-07-07 01:33:06	ID requested
⏵ 1	2026-07-07 01:34:26	announced

Dataset with its publication pending

submitter keyword: photocage, BODPIY, bioconjugation, proximity labeling, lipid droplet

Lihua Zhang
contact affiliation	Dalian Institute of Chemical Physics
contact email	lihuazhang@dicp.ac.cn
lab head
Nan Zhao
contact affiliation	Dalian Institute of Chemical Physics
contact email	zhaonan@dicp.ac.cn
dataset submitter

iProX dataset URI