PXD080642

PXD080642 is an original dataset announced via ProteomeXchange.

Title	Generation and Proteomic Analysis of Fibrin-Based 3D Engineered Vascular Tissues (EVTs) from Human Aortic Smooth Muscle Cells
Description	Vascular smooth muscle cells (SMCs) reside within the medial layer of blood vessels, where they interact with an extracellular matrix (ECM) composed of collagen, elastin, and proteoglycans to maintain vascular structure and function. Aberrant ECM remodeling contributes to multiple vascular diseases; however, conventional two-dimensional culture systems do not adequately recapitulate the three-dimensional (3D) cellular and matrix environment required to study smooth muscle cell–ECM interactions and matrix remodeling. This protocol describes the generation of engineered vascular tissues (EVTs) from primary human aortic SMCs cultured within fibrin-based 3D hydrogels. Following casting between flexible polydimethylsiloxane posts, EVTs undergo cellular alignment, contraction, and deposition of de novo ECM, providing a physiologically relevant platform for studying vascular matrix biology. The protocol details tissue fabrication, culture, harvesting, and downstream analysis of newly deposited ECM. In addition, a workflow is presented for qualitative and quantitative characterization of EVT-derived proteins using Western blotting and mass spectrometry-based proteomics. Sequential protein extraction enables assessment of soluble and ECM-enriched protein fractions, facilitating in-depth evaluation of ECM composition. This platform provides a reproducible approach for investigating ECM production and remodeling by human SMCs in a 3D environment.
HostingRepository	PRIDE
AnnounceDate	2026-08-24
AnnouncementXML	Submission_2026-08-24_04:16:07.358.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	XIAOKE YIN
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; (18)O labeled deglycosylated asparagine; iodoacetamide derivatized residue
Instrument	Orbitrap Astral Zoom

Revision	Datetime	Status	ChangeLog Entry
0	2026-07-06 23:36:19	ID requested
⏵ 1	2026-08-24 04:16:07	announced

10.3791/71784;

submitter keyword: Tissue engineering

3D cell culture;Smooth muscle cells

Extracellular matrix

Cardiovascular disease

Manuel Mayr
contact affiliation	National Heart and Lung Institute, Imperial College London, London, UK
contact email	m.mayr@imperial.ac.uk
lab head
XIAOKE YIN
contact affiliation	National Heart and Lung Institute, Faculty of Medicine, Imperial College London
contact email	xiaoke.yin@imperial.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD080642

PRIDE project URI

• PRIDE
  1. PXD080642
     1. Label: PRIDE project
     2. Name: Generation and Proteomic Analysis of Fibrin-Based 3D Engineered Vascular Tissues (EVTs) from Human Aortic Smooth Muscle Cells