PXD080522

PXD080522 is an original dataset announced via ProteomeXchange.

Title	H4K8 lactylation modulates GLS mRNA stability via ZMAT3 to induce oxidative stress-associated apoptosis and cataractogenesis
Description	Apoptosis of lens epithelial cells (LECs) is a key feature of age-related cataracts. With the aging process, the energy supply mechanism of LEC changes, resulting in impaired glucose metabolism, which in turn causes increased oxidative stress and apoptosis of cells. However, the exact intrinsic mechanism has not been fully clarified. This study shows that in the Sodium selenite-induced cataract rat model, the level of lactylation in LECs increases significantly as the disease progressed. Inhibiting lactylation can reduce cell apoptosis and delay the formation of cataracts. Specifically, the excessive lactylation of the H4K8 site directly regulates the expression of RNA-binding protein ZMAT3. After the ZMAT3 level is elevated, it can directly bind to the downstream target gene-the key glutamate metabolic enzyme GLS and regulate its mRNA stability. This leads to the downregulation of GLS expression, thus inhibiting glutamate metabolism. As a result, glutathione and other antioxidants are rapidly exhausted, reducing the antioxidant ability of LEC and eventually inducing cell apoptosis. These findings elucidate the disruption of the balance between oxidative phosphorylation and glycolysis in LECs, promoting cell apoptosis by regulating specific lactylation. This study further indicates that H4K8la and ZMAT3 are potential therapeutic targets for the prevention and treatment of age-related cataracts.
HostingRepository	iProX
AnnounceDate	2026-07-02
AnnouncementXML	Submission_2026-07-03_02:26:00.769.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hong Cheng
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2026-07-03 02:25:12	ID requested
⏵ 1	2026-07-03 02:26:01	announced

Dataset with its publication pending

submitter keyword: H4K8la, GLS mRNA stability, Cataractogenesis

Ruonan Li
contact affiliation	The First Affiliated Hospital of Chongqing Medical University
contact email	18717036067@163.com
lab head
Hong Cheng
contact affiliation	The First Affiliated Hospital of Chongqing Medical University
contact email	chenghong199797@163.com
dataset submitter

iProX dataset URI