PXD080401

PXD080401 is an original dataset announced via ProteomeXchange.

Title	Activity-Based Ubiquitin-α-Amidomethyl Acrylate Probe for Profiling of Ubiquitinating Enzymes and Deubiquitinases
Description	Activity-based ubiquitin probes have emerged as powerful tools for dissecting protein ubiquitination. In this work, we discovered that an α-amidomethyl substituted acrylate electrophile exhibits markedly enhanced reactivity. Based on this finding, we designed and synthesized a new activity-based probe, Ubiquitin-α-Amidomethyl Acrylate (Ub-AMA). In vitro biochemical assays demonstrated that Ub-AMA exhibits broad crosslinking capability across the entire ubiquitin regulatory enzyme system—including E1, E2, and E3 enzymes as well as DUBs. Compared to the classic Ub-VME probe, Ub-AMA achieves significantly higher labeling efficiency for E2 enzymes. Finally, proteomic profiling in cell lysates confirmed that Ub-AMA can capture E1, E2, and E3 enzymes as well as DUBs in a complex biological environment. In summary, the Ub-AMA probe developed here provides a new tool for in depth analysis of the dynamic regulatory mechanisms of ubiquitination. Moreover, this work offers valuable insights for the design of other activity based probes based on α,β-unsaturated carbonyl scaffolds.
HostingRepository	iProX
AnnounceDate	2026-06-30
AnnouncementXML	Submission_2026-06-30_19:29:12.952.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xu Li
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2026-06-30 19:28:50	ID requested
⏵ 1	2026-06-30 19:29:13	announced

Dataset with its publication pending

submitter keyword: Activity-Based Ubiquitin-α-Amidomethyl Acrylate Probe

Xu Li
contact affiliation	Qingdao University
contact email	lixuqd@qdu.edu.cn
lab head
Xu Li
contact affiliation	Qingdao University
contact email	lixuqd@qdu.edu.cn
dataset submitter

iProX dataset URI