⮝ Full datasets listing
PXD080383
PXD080383 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Protein Aggregation Capture for Top-down Proteomics |
| Description | Proteins are dynamically regulated by a myriad of post-translational modifications (PTMs) that control their stability, conformation, activity, subcellular localization, and local interactions. Capturing the precise composition of these various modification states, or proteoforms, is a principal objective of top-down proteomics (TDP). By ionizing intact proteoforms and combining measurements of precursor ion and fragment ion masses, the position, stoichiometry, and combination of PTMs can be determined. Despite the highly valuable measurements that TDP can provide, it is typically less sensitive than corresponding peptide-level analysis with many reports utilizing input material in the microgram to milligram range.1,2 Contributing to this lack of sensitivity is the risk of sample loss due to non-specific binding to surfaces during sample preparation. The most widely employed sample preparation approaches for TDP either require high sample input (e.g. precipitation and ultra-filtration) or fail to effectively remove surfactants (e.g. solid-phase extraction). These limitations have hindered advancement of targeted TDP applications involving immunoprecipitation and other enrichment strategies. Bead-assisted protein aggregation, also referred to as single-pot, solid-phase-enhanced sample preparation (SP3), has emerged as a popular sample preparation strategy for bottom-up proteomic workflows,3,4 but has only been used in TDP with secondary ion exchange chromatography cleanup.5 We envisioned a magnetic bead based protein cleanup approach that proceeds directly to MS analysis with judicious choice of bead surface chemistry and elution conditions. Here we report a sample preparation method using hydroxyl-functionalized magnetic beads for top-down proteomics applications. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-07-02 |
| AnnouncementXML | Submission_2026-07-02_11:42:47.692.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Isabella Feltenstein |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | Acetyl; Oxidation; Methyl; Phospho |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-06-30 10:17:55 | ID requested | |
| ⏵ 1 | 2026-07-02 11:42:48 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Proteoform, Top-down proteomics, Sample clean-up, HeLa intact protein, DatasetType:Proteomics |
Contact List
| Bryon S. Drown | |
|---|---|
| contact affiliation | Purdue University Main Campus |
| contact email | bsdrown@purdue.edu |
| lab head | |
| Isabella Feltenstein | |
| contact affiliation | Purdue University |
| contact email | ifeltens@purdue.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v13/MSV000102313/ |
