PXD080333

PXD080333 is an original dataset announced via ProteomeXchange.

Title	Discovery of peptide biomarkers in male Eupolyphaga sinensis Walker (Tubiechong) by dimethyl labeling-based quantitative peptidomics analysis
Description	Eupolyphaga sinensis Walker is a traditional Chinese medicinal insect. However, the peptide composition differences between male and female ES, which may underlie their distinct medicinal efficacies, remain largely unexplored. In this study, we performed a quantitative peptidomics analysis using stable isotope dimethyl labeling combined with high-resolution nanoLC-MS/MS to systematically compare the peptide profiles of male and female ES. Tryptic peptides from female sources were labeled with light reagents (HCHO/NaBH3CN), while those from male sources were labeled with medium reagents (DCDO/NaBH3CN). Equal amounts of labeled samples were mixed and analyzed by Q Exactive Plus mass spectrometer. The quantitative ratios of male to female peptides were calculated based on the peak area intensities of the isotope-labeled precursor ions.
HostingRepository	PRIDE
AnnounceDate	2026-09-07
AnnouncementXML	Submission_2026-09-07_04:22:15.268.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ge hui
SpeciesList	scientific name: Eupolyphaga sinensis; NCBI TaxID: NEWT:367774;
ModificationList	monohydroxylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2026-06-29 14:47:29	ID requested
⏵ 1	2026-09-07 04:22:16	announced

10.3389/fphar.2026.1799023;

Ge H, Huang X, Qing R, Liu P, Li H, Zhu J, Wei Y, Liu S, Liu X, Han S, Deng H, Liu R, Walker (Tubiechong) by dimethyl labeling-based quantitative peptidomics analysis. Front Pharmacol, 17():1799023(2026) [pubmed]

submitter keyword: Eupolyphaga sinensis；peptide biomarkers；dimethyl labeling, LC-MS/MS

Liu rui
contact affiliation	Nanjing University of Chinese Medicine
contact email	liurui@njucm.edu.cn
lab head
Ge hui
contact affiliation	Nanjing University of Chinese Medicine
contact email	202410933@njucm.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD080333
     1. Label: PRIDE project
     2. Name: Discovery of peptide biomarkers in male Eupolyphaga sinensis Walker (Tubiechong) by dimethyl labeling-based quantitative peptidomics analysis