PXD080304

PXD080304 is an original dataset announced via ProteomeXchange.

Title	Maternal IntS12 establishes a conserved Pol II checkpoint to safeguard selective zygotic genome activation
Description	Early embryos must execute faithful zygotic genome activation (ZGA), rapidly initiating transcription while preventing indiscriminate output from thousands of RNA polymerase II (Pol II)–occupied promoters. Here, we identify maternal IntS12 as a conserved genome-wide “filter” that enforces the selectivity of ZGA. In Drosophila, loss of maternal IntS12 leads to widespread activation of pseudo-ZGA genes and disruption of transcriptional hierarchy. Mechanistically, IntS12 engages IntS1 through a highly constrained N-terminal helicase-containing element to selectively stabilize promoter-proximal pausing factors (NELF) at poised loci, thereby restricting premature pause release and limiting productive elongation. Strikingly, in mouse embryos, maternal INTS12 depletion similarly causes ZGA failure and early developmental arrest. A minimal ~33–amino acid containing-two helixes from INTS12 is sufficient for this activity, functioning as a compact and highly selective module that preserves pausing integrity while suppressing ectopic transcription. Together, our findings reveal that an evolutionarily conserved microdomain containing two helixes in IntS12 acts as a Pol II checkpoint to ensure robust and selective ZGA.
HostingRepository	iProX
AnnounceDate	2026-06-29
AnnouncementXML	Submission_2026-06-29_02:11:18.194.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Guoqiang Zhang
SpeciesList	scientific name: Drosophila melanogaster; NCBI TaxID: 7227;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 240

Revision	Datetime	Status	ChangeLog Entry
0	2026-06-29 02:10:56	ID requested
⏵ 1	2026-06-29 02:11:18	announced

Dataset with its publication pending

submitter keyword: Drosophila, embryos, zygotic gene activating

Dahua Chen
contact affiliation	Institute of Biomedical Research, Yunnan University
contact email	chendh@ynu.edu.cn
lab head
Guoqiang Zhang
contact affiliation	Institute Of Biomedical Research,Yunnan University
contact email	gqzhang@ynu.edu.cn
dataset submitter

iProX dataset URI