PXD080227

PXD080227 is an original dataset announced via ProteomeXchange.

Title	Depletion of CARM1 slows in-vivo tumorigenicity and rewires developmental programming in Glioma stem-like cells
Description	Glioma stem-like cells (GSCs) exploit developmental signaling programs that contribute to glioblastoma heterogeneity and therapy resistance. Here, we define a role for the arginine methyltransferase CARM1 in regulating GSC lineage state and survival signaling. CARM1 depletion slows GSC growth, increases apoptosis, alters histone post-translational modifications, and shifts transcriptomic and proteomic profiles toward a radial glial-like state. Loss of CARM1 increases NGFR/NTRK signaling and sensitizes GSCs to NTRK and AKT inhibition. Mechanistically, NFIA is a CARM1 substrate, and mutation of NFIA arginine 389 increases NGFR expression, supporting a role for CARM1-dependent NFIA methylation in NGFR repression. In orthotopic xenografts, CARM1 depletion reduces tumor burden and prolongs survival. These findings identify CARM1 as a regulator of GSC developmental programs and NGFR/NTRK-dependent survival.
HostingRepository	PRIDE
AnnounceDate	2026-06-28
AnnouncementXML	Submission_2026-06-28_10:09:07.423.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Simone Sidoli
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos; timsTOF HT

Revision	Datetime	Status	ChangeLog Entry
0	2026-06-26 07:13:16	ID requested
⏵ 1	2026-06-28 10:09:07	announced

Dataset with its publication pending

submitter keyword: NGFR, histones, AKT, Entrectinib,Glioblastoma, ERK, CARM1, proteomics, radial glial cell, development, arginine methylation, NFIA, NTRK, glioma stem-like cell, chromatin

Simone Sidoli
contact affiliation	Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461, United States
contact email	simone.sidoli@einsteinmed.edu
lab head
Simone Sidoli
contact affiliation	Albert Einstein College of Medicine
contact email	simone.sidoli@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD080227

PRIDE project URI

• PRIDE
  1. PXD080227
     1. Label: PRIDE project
     2. Name: Depletion of CARM1 slows in-vivo tumorigenicity and rewires developmental programming in Glioma stem-like cells