⮝ Full datasets listing
PXD080211
PXD080211 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Structural and mechanistic insights into a phage NAD reconstitution pathway mediated by a high energy ADPR PP |
| Description | NAD depletion is a widespread bacterial defense strategy against phage infection. In turn, phages have evolved NAD reconstitution pathways (NARPs) to replenish NAD and sustain infection. However, the molecular basis underlying NARP function remains poorly understood. Here, we uncover the structural and mechanistic basis of ADP ribose pyrophosphate synthetase (Adps) and nicotinamide ADPR transferase (Namat) in the NARP1 pathway. Structural snapshots of Adps reveal a dramatic 22 A catalytic loop closure that recognizes the adenine moiety of ADPR and simultaneously drives active-site completion and intermediate stabilization. Adps structures bound to ATP and ADPR analogs support a sequential Bi Bi mechanism in which ATP binding primes subsequent ADPR recognition, followed by ordered AMP and ADPR-PP release. Adps lacks allosteric sites for ADP inhibition, but instead assembles with Namat containing a distinct insertion domain into higher order complexes that facilitate NAD reconstitution. Overall, these findings advance our understanding of NAD synthesis through NARP1 pathway and the bacterial phage arms race |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-06-26 |
| AnnouncementXML | Submission_2026-06-26_02:04:03.590.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | cjchen |
| SpeciesList | scientific name: Erwinia amylovora phage; scientific name: Shigella phage vB; |
| ModificationList | CarbamidomethylDTT |
| Instrument | timsTOF HT |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-06-26 00:59:45 | ID requested | |
| ⏵ 1 | 2026-06-26 02:04:04 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: NAD+, phage NAD+ reconstitution pathway, ADPR-PP, ADPR-PP synthetase (Adps), nicotinamide ADPR transferase (Namat), DatasetType:Proteomics |
Contact List
| Chao-Jung Chen | |
|---|---|
| contact affiliation | China Medical University |
| contact email | cjchen@mail.cmu.edu.tw |
| lab head | |
| cjchen | |
| contact affiliation | China Medical University |
| contact email | cjchen@mail.cmu.edu.tw |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v13/MSV000102279/ |
