⮝ Full datasets listing
PXD080188
PXD080188 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Differential Interaction Analysis by LC-MS: Plasmodium falciparum HSP90 co-IP |
| Description | The Duke Proteomics and Metabolomics Core Facility (DPMCF) received 6 samples total which were kept at -80C until processing. Samples were spiked with 1 or 2 pmol bovine casein as an internal digestion quality control standard. Next, they were brought to 5% SDS and reduced for 15min at 80C, alkylated with 20mM iodoacetamide for 30min at room temperature, then supplemented with a final concentration of 1.2% phosphoric acid and 695uL of S-Trap (Protifi) binding buffer (90% MeOH/100mM TEAB). Proteins were trapped on the S-Trap micro cartridge, digested using 1ug sequencing grade trypsin (Promega) for 1 hr at 47C, and eluted using 50 mM TEAB, followed by 0.2% FA, and lastly using 50% ACN/0.2% FA. All samples were then lyophilized to dryness. Samples were resuspended in 200uL of 0.1% formic acid. A study pool QC (SPQC) was created by combining equal volumes of each sample and run periodically throughout the study. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-08-31 |
| AnnouncementXML | Submission_2026-08-31_08:02:20.153.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Jeffrey Kuhn |
| SpeciesList | scientific name: Plasmodium falciparum 3D7; NCBI TaxID: 36329; scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Astral |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-06-25 12:45:37 | ID requested | |
| ⏵ 1 | 2026-08-31 08:02:20 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: HSP90, DatasetType:Proteomics |
Contact List
| Emily Derbyshire, PhD | |
|---|---|
| contact affiliation | Duke University |
| contact email | emily.derbyshire@duke.edu |
| lab head | |
| Jeffrey Kuhn | |
| contact affiliation | Duke University |
| contact email | jfk31@duke.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v13/MSV000102274/ |
