PXD080161

PXD080161 is an original dataset announced via ProteomeXchange.

Title	4D-DIA proteomics of Nicotiana benthamiana leaves under mechanical (buffer), Agrobacterium, and recombinant-cargo (GFP / FMDV P1_2A) stress
Description	Time-resolved 4D-DIA (data-independent acquisition with trapped ion mobility) proteomic profiling of Nicotiana benthamiana leaves to dissect host responses during transient expression. Five treatment groups — untreated wild type (WT), buffer infiltration (mechanical stress), empty-vector Agrobacterium GV3101 (bacterial stress), and Agrobacterium expressing GFP or the Foot-and-Mouth Disease Virus antigen P1_2A (product-specific stress) — were sampled at 1, 4 and 7 days post-infiltration (dpi), with three biological replicates per condition (45 samples). Data acquired on a Bruker timsTOF Pro in 4D-DIA mode and analysed with DIA-NN (library-free).
HostingRepository	iProX
AnnounceDate	2026-06-24
AnnouncementXML	Submission_2026-08-14_02:33:13.427.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yiming Sun
SpeciesList	scientific name: Nicotiana benthamiana; NCBI TaxID: 4100;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2026-06-25 03:56:17	ID requested
⏵ 1	2026-08-14 02:33:13	announced

Dataset with its publication pending

submitter keyword: Nicotiana benthamiana, agroinfiltration, molecular farming, 4D-DIA, timsTOF, proteomics, protease, unfolded protein response, virus-like particle, recombinant protein

Lijun Zhu
contact affiliation	Chongqing Key Laboratory of Scientific Utilization of Tobacco Resources
contact email	zhulj7802@163.com
lab head
Yiming Sun
contact affiliation	Southwest University
contact email	s8355@swu.edu.cn
dataset submitter

iProX dataset URI