PXD080160

PXD080160 is an original dataset announced via ProteomeXchange.

Title	Macrophage blebs mediate functional mitochondrial transfer and collagen biomineralization
Description	Critical bone defects pose substantial clinical challenges due to the early hypoxic and inflammatory niche that traps macrophages in a glycolytic state and bottlenecks regeneration. Here, we developed a zwitterionic oxygen-generating scaffold (ZHM@CaO2) that rescues macrophage mitochondrial respiration, driving robust anti-inflammatory M2 polarization. Strikingly, rather than acting merely as indirect signaling hubs, these revived macrophages assume an unprecedented role as direct matrix builders by actively shedding blebs. Fueled by encapsulated, functionally intact donor mitochondria, these autonomous blebs execute energy-demanding tasks independently within the extracellular space. Mechanistically, they exert dual regenerative functions: dictating recipient bone marrow stem cell (BMSC) osteogenesis via intercellular mitochondrial transfer, and directly interacting with the extracellular matrix to catalyze intrafibrillar collagen mineralization, with surface-bound calreticulin (CALR) serving as a macromolecular nucleating template. By demonstrating that immune-derived blebs can directly drive skeletal biomineralization, this study expands the traditional paradigm of bone healing and provides a bioenergetic blueprint for next-generation bio-instructive biomaterials.
HostingRepository	iProX
AnnounceDate	2026-06-24
AnnouncementXML	Submission_2026-06-29_00:08:56.959.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bicong Gao
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2026-06-25 03:38:54	ID requested
⏵ 1	2026-06-29 00:08:57	announced

Dataset with its publication pending

submitter keyword: macrophage, bone, mitochondria, microvesicles

Wenjin Cai
contact affiliation	Zhejiang University
contact email	7322019@zju.edu.cn
lab head
Bicong Gao
contact affiliation	Zhejiang University
contact email	3150103153@zju.edu.cn
dataset submitter

iProX dataset URI