⮝ Full datasets listing

PXD080137

PXD080137 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe study on 6 -MDS Proteomics
DescriptionThe tumor tissues of nude mice were used as research materials for differential protein analysis. The samples were ground in liquid nitrogen, lysed by shaking, crushed by sonication, and the supernatant was removed for protein quantification. Dithiothreitolwas added to the sample and incubated. Iodoacetamide was added to the sample and incubated. Then trypsin was added at a ratio of 1:50 and digested. 1 mL of protein lysate was loaded, washed with formic acid water, eluted with acetonitrile, and then stored at -20 ° C after vacuum drying using a bench top cryo-vacuum concentrator. Marker peptides by high performance liquid mixture separation into different components, detection wavelength of 214 nm, liquid flow rate 0.7 mL·min - 1, gradient elution and collect 12 peptides outflow of components, and merge as mass spectrum identification of eight components. On the sample of the sample after through the analysis of column elution into Q Exactive HF combined mass spectrometry identification, sample use UltiMate3000 upgrade high performance liquid chromatography system. The mass spectrum data files use MaxQuant (v. 1.6.10.43, https://maxquant.org/) search engine search and use the Uniprot database search library (https://www.uniprot.org/) protein, Functional annotation and enrichment analysis were performed for the differentially expressed proteins.
HostingRepositoryiProX
AnnounceDate2026-06-23
AnnouncementXMLSubmission_2026-06-29_00:13:32.310.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLei Zhang
SpeciesList scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02026-06-24 18:47:23ID requested
12026-06-29 00:13:32announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: 6 -MDS Proteomics
Contact List
Zengjun Guo
contact affiliationXi'an Jiaotong University
contact email3131675459@163.com
lab head
Lei Zhang
contact affiliationShaanxi Provincial Hospital of Traditional Chinese Medicine
contact emailaralezhanglei@163.com
dataset submitter
Full Dataset Link List
iProX dataset URI