PXD080067

PXD080067 is an original dataset announced via ProteomeXchange.

Title	Whole-proteome profiling of human lung cancer brain metastasis-associated fibroblasts
Description	Cancer-associated fibroblasts (CAFs) are increasingly recognized as an important component of the brain metastasis tumour microenvironment; however, their proteomic phenotype remains poorly characterized. This dataset contains mass spectrometry-based proteomic profiles of primary human brain metastasis-associated fibroblasts (BrM-CAFs) isolated from lung cancer brain metastases (L-BrM-CAF, n = 3) and control, non-tumorous subgalear fibroblasts (Subgalear-NF, n = 3). Whole-cell lysates were analyzed using data-independent acquisition (DIA) mass spectrometry, supported by a project-specific spectral library generated from data-dependent acquisition (DDA) measurements. The dataset was generated to characterize the molecular phenotype of BrM-CAFs and to investigate protein-level alterations associated with stromal activation, extracellular matrix remodelling, and tumour–stroma interactions. The data provide a resource for studies of the brain metastasis microenvironment and CAF biology.
HostingRepository	PRIDE
AnnounceDate	2026-08-08
AnnouncementXML	Submission_2026-08-08_14:36:16.985.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jakub Cervenka
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF HT

Revision	Datetime	Status	ChangeLog Entry
0	2026-06-23 08:43:42	ID requested
1	2026-08-03 03:02:19	announced
⏵ 2	2026-08-08 14:36:17	announced	2026-08-08: Updated project metadata.

V, ý, molov, á B, Pfeiferov, á L, Smetana TK, V, ý, mola P, Ternerov, á N, Zuba, ľ M, Kol, á, ř M, Š, á, chov, á J, Gy, ö, ny, ö, rov, á S, Bal, á, ž, iov, á E, Houdov, á, Megov, á M, Č, ervenka J, Kupcov, á, Skaln, í, kov, á H, Š, panko M, Smetana K, Lacina L, Netuka D, Tom, á, š R, Bu, š, ek P, Š, edo A, Brain metastasis-associated fibroblasts shape the tumour microenvironment to enhance cancer cell invasion. Brain Pathol, ():e70129(2026) [pubmed]

10.1111/bpa.70129;

submitter keyword: primary cell culture, cell migration, LC-MS,brain metastases, cancer-associated fibroblasts, extracellular matrix

Helena Kupcová Skalníková
contact affiliation	Laboratory of Proteomics, Institute of Biochemistry and Experimental Oncology, First Faculty of Medicine, Charles University, U Nemocnice 5, Prague 2, 128 00, Czech Republic
contact email	helena.skalnikova@lf1.cuni.cz
lab head
Jakub Cervenka
contact affiliation	Laboratory of Proteomics, Institute of Biochemistry and Experimental Oncology, First Faculty of Medicine, Charles University, Prague, Czech Republic
contact email	jakub.cervenka@lf1.cuni.cz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD080067

PRIDE project URI

• PRIDE
  1. PXD080067
     1. Label: PRIDE project
     2. Name: Whole-proteome profiling of human lung cancer brain metastasis-associated fibroblasts