PXD080046

PXD080046 is an original dataset announced via ProteomeXchange.

Title	Proximity-dependent TurboID labeling screen on the LAMTOR complex
Description	This proximity-dependent labeling screen was set up to investigate the “proxisome” (i.e. the sum of proteins in the labeling diameter around a bait protein) of the LAMTOR complex. Furthermore, we aimed at understanding the recruitment dynamics of known and unknown proteins to and from the LAMTOR complex upon changing environmental conditions. Therefore, cells were exposed to three different conditions: Full medium (FM), amino acid and growth factor starvation (EBSS) and replenishment of amino acid and growth factors after starvation (HF). To achieve that, we generated stable cell lines expressing the biotin ligase TurboID fused to two LAMTOR subunits (LAMTOR3 and LAMTOR5) as well as to a lysosomal (TMEM192) and cytosolic (nuclear exclusion sequence NES) control. After induction of the biotin-ligase reaction in living cells, biotinylated proteins were extracted from cell lysates via streptavidin beads and further processed for mass spectrometry analysis.
HostingRepository	PRIDE
AnnounceDate	2026-07-10
AnnouncementXML	Submission_2026-07-10_03:16:07.946.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Markus Hartl
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2026-06-23 02:19:22	ID requested
⏵ 1	2026-07-10 03:16:08	announced

Dataset with its publication pending

submitter keyword: LC-MSMS, FlpIn-T-Rex 293 cells, Human, LAMTOR baits, embryonic kidney,TurboID

Prof. Manuela Baccarini
contact affiliation	Max-Perutz Labs/ Mechanistic Cell and Developmental Biology/University of Vienna, Dr.-Bohr-Gasse 9, 1030 Vienna, Austria
contact email	Manuela.baccarini@univie.ac.at
lab head
Markus Hartl
contact affiliation	Max Perutz Labs, Mass Spectrometry Facility, Vienna Biocenter Campus (VBC), Dr. Bohr-Gasse 9, Vienna, Austria
contact email	proteomics@maxperutzlabs.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD080046

PRIDE project URI

• PRIDE
  1. PXD080046
     1. Label: PRIDE project
     2. Name: Proximity-dependent TurboID labeling screen on the LAMTOR complex