PXD079978

PXD079978 is an original dataset announced via ProteomeXchange.

Title	AQP9 Drives Myocardial Ferroptosis via TRIM25-Mediated GPX4 Ubiquitination and PI3K/AKT Suppression
Description	This proteomics dataset investigates the role of aquaporin-9 (AQP9) in regulating ferroptosis during myocardial ischemia-reperfusion (I/R) injury. Quantitative proteomic analysis of H9c2 cardiomyocytes (shNC controls vs shAQP9 knockdowns) was performed on a Bruker timsTOF, and AQP9 immunoprecipitation-mass spectrometry (IP-MS) was acquired on a Thermo Q Exactive HF. The data reveal that AQP9 drives ferroptotic cell death by promoting GPX4 degradation via TRIM25-mediated ubiquitination while simultaneously inhibiting GPX4 synthesis through the PI3K/AKT pathway, thereby dismantling antioxidant defenses in ischemic cardiomyocytes.
HostingRepository	iProX
AnnounceDate	2026-06-22
AnnouncementXML	Submission_2026-06-22_01:34:18.838.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jianye Yang
SpeciesList	scientific name: Rattus norvegicus; NCBI TaxID: 10116;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2026-06-22 01:33:21	ID requested
⏵ 1	2026-06-22 01:34:19	announced

Dataset with its publication pending

submitter keyword: Aquaporin 9, Ferroptosis,GPX4, TRIM25,PI3K/AKT, Myocardial ischemia-reperfusion

Jianye Yang
contact affiliation	Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology
contact email	jianye_yang@hust.edu.cn
lab head
Jianye Yang
contact affiliation	Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology
contact email	jianye_yang@hust.edu.cn
dataset submitter

iProX dataset URI